Neuroblast proliferation on the surface of the adult rat striatal wall after focal ependymal loss by intracerebroventricular injection of neuraminidase
Neuroblast proliferation on the surface of the adult rat striatal wall after focal ependymal loss by intracerebroventricular injection of neuraminidase
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DOI：
10.1002/cne.21618
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发表时间：
2008-04-01
影响因子：
2.5
通讯作者：
Fernandez-Llebrez, Pedro
中科院分区：
文献类型：
--
作者：
Gomez-Roldan, Maria Del Carmen;Perez-Martin, Margarita;Fernandez-Llebrez, Pedro
成年啮齿动物纹状体壁的脑室下区是生命活动的神经源性区域。立方多纤毛室管膜将脑室下区与脑脊液(CSF)分开，参与成人神经发生的调控。将产气荚膜梭菌神经氨酸酶注入大鼠右侧脑室，可引起纹状体室管膜部分脱离。对侧脑室未受影响，作为实验对照。神经氨酸酶导致一些室管膜细胞间的间隙变宽，随后脑脊液中室管膜细胞脱落和崩解。脑室管膜部分剥离后，局部脉络丛巨噬细胞和中性粒细胞渗入脑脊液，注射神经氨酸酶后室管膜细胞从未脱离。炎症向脑室周围实质扩散。室管膜细胞不分离，留在室壁内，从未增殖。丢失的室管膜再也没有被修复，室管膜细胞也从未表现出神经干细胞的行为。相反，由重叠的星形细胞突起形成的疤痕封闭了那些没有室管膜的区域。剥脱区边缘的一些室管膜细胞与脑室失去联系，并位于胶质层之下。伴随着瘢痕的形成，一些室管膜下细胞通过室管膜裂隙向脑室突起，增殖，形成圆形的脑室细胞簇，表达成神经母细胞的表型。注射后90天，脑室内仍有成簇的神经母细胞。在脑室下区，成体神经发生持续存在。
The subventricular zone of the striatal wall of adult rodents is an active neurogenic region for life. Cubic multiciliated ependyma separates the subventricular zone from the cerebrospinal fluid (CSF) and is involved in the control of adult neurogenesis. By injecting neuraminidase from Clostridium perfringens into the right lateral ventricle of the rat, we provoked a partial detachment of the ependyma in the striatal wall. The contralateral ventricle was never affected and was used as the experimental control. Neuraminidase caused widening of the intercellular spaces among some ependymal cells and their subsequent detachment and disintegration in the CSF. Partial ependymal denudation was followed by infiltration of the CSF with macrophages and neutrophils from the local choroid plexus, which ependymal cells never detached after neuraminidase administration. Inflammation extended toward the periventricular parenchyma. The ependymal cells that did not detach and remained in the ventricle wall never proliferated. The lost ependyma was never recovered, and ependymal cells never behaved as neural stem cells. Instead, a scar formed by overlapping astrocytic processes sealed those regions devoid of ependyma. Some ependymal cells at the border of the denudated areas lost contact with the ventricle and became located under the glial layer. Concomitantly with scar formation, some subependymal cells protruded toward the ventricle through the ependymal breaks, proliferated, and formed clusters of rounded ventricular cells that expressed the phenotype of neuroblasts. Ventricular clusters of neuroblasts remained in the ventricle up to 90 days after injection. In the subventricular zone, adult neurogenesis persisted.