Genetic analysis of the Drosophila 63F early puff. Characterization of mutations in E63-1 and maggie, a putative Tom22.
Genetic analysis of the Drosophila 63F early puff. Characterization of mutations in E63-1 and maggie, a putative Tom22.
复制标题
果蝇 63F 早期泡芙的遗传分析。
DOI：
10.1093/genetics/156.1.229
复制
发表时间：
2000
期刊：
影响因子：
3.3
通讯作者：
Andres,AJ
中科院分区：
文献类型：
--
作者：
Vaskova,M;Bentley,AM;Marshall,S;Reid,P;Thummel,CS;Andres,AJ
幼虫唾液腺多线染色体中的63F早期膨体含有不同转录的E63-1和E63-2蜕皮激素诱导基因。E63-1编码Ca2+结合蛋白EF-hand家族的成员，而E63-2没有明显的开放阅读框。为了了解E63基因的功能，我们确定了E63-1蛋白表达的时间和空间模式，并对63F泡芙进行了遗传分析。我们发现 E63-1 在许多胚胎和幼虫组织中表达，但三龄幼虫唾液腺是唯一蛋白质水平增加与蜕皮激素滴度增加相关的组织。此外，在变态开始时，E63-1蛋白的亚细胞分布在唾液腺中动态变化。然而，E63-1和E63-2无效突变对发育或生育力没有影响。我们已经表征了 63F 区域的 40 kb，定义为 Ubi-pandE63-2 之间的间隔，并鉴定了对应于 thedSc-2、ida 和 mgegenes 的三个致死互补组。我们发现 mge 突变会导致一龄幼虫致死，并且 Mge 蛋白与真菌的 Tom22 线粒体输入蛋白相似，表明它在线粒体功能中发挥作用。
The 63F early puff in the larval salivary gland polytene chromosomes contains the divergently transcribedE63-1andE63-2ecdysone-inducible genes.E63-1encodes a member of the EF-hand family of Ca2+-binding proteins, whileE63-2has no apparent open reading frame. To understand the functions of theE63genes, we have determined the temporal and spatial patterns of E63-1 protein expression, as well as undertaken a genetic analysis of the 63F puff. We show that E63-1 is expressed in many embryonic and larval tissues, but the third-instar larval salivary gland is the only tissue where increases in protein levels correlate with increases in ecdysone titer. Furthermore, the subcellular distribution of E63-1 protein changes dynamically in the salivary glands at the onset of metamorphosis.E63-1andE63-2null mutations, however, have no effect on development or fertility. We have characterized 40 kb of the 63F region, defined as the interval betweenUbi-pandE63-2, and have identified three lethal complementation groups that correspond to thedSc-2, ida, andmgegenes. We show thatmgemutations lead to first-instar larval lethality and that Mge protein is similar to the Tom22 mitochondrial import proteins of fungi, suggesting that it has a role in mitochondrial function.