Comparison of the repeatability of GABA-edited magnetic resonance spectroscopy with and without macromolecule suppression
Comparison of the repeatability of GABA-edited magnetic resonance spectroscopy with and without macromolecule suppression
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DOI：
10.1002/mrm.25699
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发表时间：
2016-03-01
影响因子：
3.3
通讯作者：
Evans, C. John
中科院分区：
文献类型：
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作者：
Mikkelsen, Mark;Singh, Krish D.;Evans, C. John
目的利用编辑磁共振波谱（MRS）技术可以在活体内对抑制性神经递质氨基丁酸（GABA）进行定量分析，但定量分析受到大分子（MM）的干扰。使用对称编辑可以抑制这种污染，但这种方法可能会损害GABA测量的可靠性。本研究的目的是比较的重复性GABA编辑MRS与MM suppress.MethodsGABA（非MM污染）和GABA+MM（MM污染）浓度测定在枕叶（OCC）和前扣带回（AC）使用对称和标准编辑（n = 15）。每个方法进行了两次，在每个region. ResultsWithin参与者的变异系数为每种技术分别为4.0%（GABA+MM）和8.6%（GABA）的OCC和14.8%（GABA+MM）和12.6%（GABA）的AC。在OCC（0.72 vs 0.67）和AC（0.41 vs 0.16）中，抑制方法的组内相关系数优于标准编辑。这些发现被复制在OCC的第二个队列（n = 15）。ConclusionSymmetric suppression被证明是可比的重复性标准GABA编辑。随着对GABA浓度、病理学和健康行为之间联系的研究越来越多，测量更纯的GABA定量变得越来越重要。Magn Reson Med 75：946-953，2016年。(c)2015 Wiley Periodicals，Inc.
PurposeThe inhibitory neurotransmitter -aminobutyric acid (GABA) can be measured in vivo using edited magnetic resonance spectroscopy (MRS), but quantification suffers from contamination by macromolecules (MM). It is possible to suppress this contamination using symmetric editing, but this procedure potentially compromises reliability of the GABA measurement. The aim of this study was to compare the repeatability of GABA-edited MRS with and without MM suppression.MethodsGABA (non-MM contaminated) and GABA+MM (MM-contaminated) concentration was measured in the occipital lobe (OCC) and anterior cingulate (AC) using symmetric and standard editing (n = 15). Each method was performed twice in each region.ResultsWithin-participant coefficients of variation for each technique were 4.0% (GABA+MM) and 8.6% (GABA) in the OCC and 14.8% (GABA+MM) and 12.6% (GABA) in the AC. Intraclass correlation coefficients were better for the suppression method than standard editing in both the OCC (0.72 versus 0.67) and AC (0.41 versus 0.16). These findings were replicated in the OCC of a second cohort (n = 15).ConclusionSymmetric suppression is shown to be comparable in repeatability to standard GABA-editing. Measuring a purer quantification of GABA becomes increasingly important as more research is conducted on links between GABA concentration, pathology and healthy behavior. Magn Reson Med 75:946-953, 2016. (c) 2015 Wiley Periodicals, Inc.