ACOUSTIC MICROSCOPY OF LIVING CELLS
ACOUSTIC MICROSCOPY OF LIVING CELLS
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DOI：
10.1073/pnas.78.3.1656
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
QUATE, CF
中科院分区：
文献类型：
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作者：
HILDEBRAND, JA;RUGAR, D;QUATE, CF
本文报道了体外活细胞[鸡胚心室]声学显微镜观察的初步结果。扫描声学显微镜使用高频声波产生亚微米分辨率的图像。在活细胞的声学显微照片中观察到的对比度取决于细胞的声学特性(即密度、硬度和衰减)和细胞的地形轮廓。声学透镜和被观察细胞之间的距离的变化也对图像产生了深远的影响。当底物位于焦平面时，细胞的厚区表现出与细胞声衰减(细胞质粘度的函数)有关的变暗。当单元格的顶部放置在焦平面附近时，图像中会出现同心的亮环和暗环。环的位置可以与细胞的拓扑结构有关，而环的对比度可以与细胞的硬度和密度相关。当单个细胞生长的基质改变为不同的材料时，它们的图像特征会有很大的变化。通过仔细选择衬底，可以增加声像的信息量。对活跃的运动细胞的声像分析表明，前导细胞的致密或僵硬程度低于细胞的静止拖尾过程。
This paper reports preliminary results of the observation by acoustic microscopy of living cells [embryonic chicken heart ventricles] in vitro. The scanning acoustic microscope uses high-frequency sound waves to produce images with submicrometer resolution. The contrast observed in acoustic micrographs of living cells depends on the acoustic properties (i.e., density, stiffness and attenuation) and on the topographic contour of the cell. Variation in distance separating the acoustic lens and the viewed cell also has a profound effect on the image. When the substratum is located at the focal plane, thick regions of the cell show a darkening that can be related to cellular acoustic attenuation (a function of cytoplasmic viscosity). When the top of the cell is placed near the focal plane, concentric bright and dark rings appear in the image. The location of the rings can be related to cell topography, and the ring contrast can be correlated to the stiffness and density of the cell. The character of the images of single cells varies dramatically when the substratum upon which they are grown is changed to a different material. By careful selection of the substratum, the information content of the acoustic images can be increased. Analysis of acoustic images of actively motile cells indicates that leading lamella are less dense or stiff than the quiescent trailing processes of the cells.