Chemogenetic manipulation of microglia inhibits neuroinflammation and neuropathic pain in mice.
Chemogenetic manipulation of microglia inhibits neuroinflammation and neuropathic pain in mice.
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DOI：
10.1016/j.bbi.2020.11.030
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Wu LJ
中科院分区：
文献类型：
--
作者：
Yi MH;Liu YU;Liu K;Chen T;Bosco DB;Zheng J;Xie M;Zhou L;Qu W;Wu LJ
Microglia play an important role in the central sensitization and chronic pain. However, a direct connection between microglial function and pain development in vivo remains incompletely understood. To address this issue, we applied chemogenetic approach by using CX3CR1creER/+:R26LSL-hM4Di/+ transgenic mice to enable expression of inhibitory Designer Receptors Exclusively Activated by Designer Drugs (Gi DREADD) in microglia. We found that microglial Gi DREADD activation inhibited spinal nerve transection (SNT)-induced microglial reactivity as well as chronic pain in both male and female mice. Gi DREADD activation downregulated the transcription factor interferon regulatory factor 8 (IRF8) and its downstream target pro-inflammatory cytokine interleukin 1 beta (IL-1β). Using in vivo spinal cord recording, we found that activation of microglial Gi DREADD attenuated synaptic transmission following SNT. Our results demonstrate that microglial Gi DREADD reduces neuroinflammation, synaptic function and neuropathic pain after SNT. Thus, chemogenetic approaches provide a potential opportunity for interrogating microglial function and neuropathic pain treatment.
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DOI：
10.1073/pnas.0700293104
发表时间：
2007-03-20
影响因子：
11.1
作者：
Armbruster, Blaine N.;Li, Xiang;Roth, Bryan L.
通讯作者：
Roth, Bryan L.
小胶质细胞接触可防止过度的去极化，并挽救神经元的兴奋性毒性。
影响因子：
3.4
作者：
Kato G;Inada H;Wake H;Akiyoshi R;Miyamoto A;Eto K;Ishikawa T;Moorhouse AJ;Strassman AM;Nabekura J
通讯作者：
Nabekura J
影响因子：
5.5
作者：
Agulhon, Cendra;Boyt, Kristen M.;McCarthy, Ken D.
通讯作者：
McCarthy, Ken D.
影响因子：
5.3
作者：
Jung, S;Aliberti, J;Littman, DR
通讯作者：
Littman, DR
影响因子：
3
作者：
Ilg AK;Enkel T;Bartsch D;Bähner F
通讯作者：
Bähner F