Photocrosslinked nanocomposite hydrogels from PEG and silica nanospheres: Structural, mechanical and cell adhesion characteristics
Photocrosslinked nanocomposite hydrogels from PEG and silica nanospheres: Structural, mechanical and cell adhesion characteristics
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DOI：
10.1016/j.msec.2012.12.099
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发表时间：
2013-04-01
影响因子：
7.9
通讯作者：
Schmidt, Gudrun
中科院分区：
文献类型：
--
作者：
Gaharwar, Akhilesh K.;Rivera, Christian;Schmidt, Gudrun
光聚合水凝胶被广泛研究用于各种组织工程应用，主要是由于它们以微创方式形成水凝胶的能力。虽然可光交联的水凝胶提供了必要的生物和化学特性来模拟细胞微环境，但它们通常缺乏足够的机械性能。最近，纳米复合材料方法已证明有可能通过增强水凝胶网络来克服这些缺陷。在这项研究中，我们研究了光交联聚乙二醇（PEG）-二氧化硅水凝胶的一些物理，化学和生物学性质。二氧化硅纳米球的加入显著抑制了PEG水凝胶的水合程度，表明二氧化硅纳米球和聚合物链之间的表面相互作用。没有观察到由于二氧化硅纳米球的加入而导致的水凝胶微观结构或平均孔径的显著变化。然而，二氧化硅纳米球的添加显著增加了水凝胶网络的机械强度和韧性。通过在水凝胶表面接种成纤维细胞来评价这些纳米复合水凝胶的生物学性质。虽然PEG水凝胶显示出最小的细胞粘附、铺展和增殖，但二氧化硅纳米球的添加增强了初始细胞粘附，促进了细胞铺展并增加了细胞的代谢活性。总体而言，结果表明，二氧化硅纳米球的添加改善了PEG水凝胶的机械刚度和细胞粘附性能，并且可用于需要受控细胞粘附的生物医学应用。(C)2013年由Elsevier B. V.出版
Photopolymerized hydrogels are extensively investigated for various tissue engineering applications, primarily due to their ability to form hydrogels in a minimally invasive manner. Although photocrosslinkable hydrogels provide necessary biological and chemical characteristics to mimic cellular microenvironments, they often lack sufficient mechanical properties. Recently, nanocomposite approaches have demonstrated potential to overcome these deficits by reinforcing the hydrogel network with. In this study, we investigate some physical, chemical, and biological properties of photocrosslinked poly(ethylene glycol) (PEG)-silica hydrogels. The addition of silica nanospheres significantly suppresses the hydration degree of the PEG hydrogels, indicating surface interactions between the silica nanospheres and the polymer chains. No significant change in hydrogel microstructure or average pore size due to the addition of silica nanospheres was observed. However, addition of silica nanospheres significantly increases both the mechanical strength and the toughness of the hydrogel networks. The biological properties of these nanocomposite hydrogels were evaluated by seeding fibroblast cells on the hydrogel surface. While the PEG hydrogels showed minimum cell adhesion, spreading and proliferation, the addition of silica nanospheres enhanced initial cell adhesion, promoted cell spreading and increased the metabolic activity of the cells. Overall, results indicate that the addition of silica nanospheres improves the mechanical stiffness and cell adhesion properties of PEG hydrogels and can be used for biomedical applications that required controlled cell adhesion. (C) 2013 Published by Elsevier B.V.