Comparative analysis of the genomes of the temperate bacteriophages φ11, φ12 and φ13 of Staphylococcus aureus 8325
Comparative analysis of the genomes of the temperate bacteriophages φ11, φ12 and φ13 of Staphylococcus aureus 8325
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DOI：
10.1016/s0378-1119(02)00481-x
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发表时间：
2002-05-01
期刊：
影响因子：
3.5
通讯作者：
Roe, BA
中科院分区：
文献类型：
--
作者：
Iandolo, JJ;Worrell, V;Roe, BA
从序列数据库中提取了金黄色葡萄球菌8325染色体上所含的三种温性噬菌体的基因组并进行了分析。φ 11、φ 12和φ 13是同一裂解群但不同血清群的成员，因此共同生活在同一宿主细胞中。它们的基因组约为42 kb至45 kb，包含至少50个密码子的约90个ORF。其中，约50个与已知基因或其他葡萄球菌的基因相似。同源组内的每个同源簇共享大的序列同一性区域，而组间同源性相对较低。基因在三个噬菌体染色体上的排列是相似的，并且与目前噬菌体基因组织的模块化理论相一致。复制的基因组似乎是由不同的机制包装的。已发现噬菌体phi 11和phi 12含有与pac-位点pac一致的序列，而phi 13具有与cos-位点pac一致的序列。而phi 12和phi 13整合到特定基因中。phi 12的att-site位于一个未知基因内，而phi 13的att-site位于β-毒素基因内。与其他两个基因相比，phi 13还引入了葡激酶基因（sak）和与fib表达相关的第二个基因。(C)2002 Elsevier Science B. V.保留所有权利。
The genomes of the three temperate bacteriophages contained in the chromosome of Staphylococcus aureus 8325 have been extracted from the sequence database and analyzed. phi 11, phi 12 and phi 13 are members of the same lytic group but different serogroups and consequently co-habitate the same host cell. Their genomes are approximately 42 kb to 45kb and contain about 90 ORFs of at least 50 codons. Of these, about 50 have similarities to known genes or to genes of other staphylococcal phages. Each of the phages clusters within a homology group that share large regions of sequence identity while intergroup homology is comparatively low. The arrangement of genes on the chromosomes of the three phages is similar and consistent with current modular theory of phage gene organization. The replicated genomes appear to be packaged by different mechanisms. Phage phi11 and phi 12 have been found to contain sequences consistent with pac-site phages while phi13 has sequences consistent with cos-site phages, The attB site for phi11 is located in an intergenic region of the S. aureus chromosome while 12 and phi13 integrate into specific genes. The phi12 att-site is within an unknown gene, but the phi13 att-site is within the beta-toxin gene. In contrast to the other two phages, phi13 also introduces the staphylokinase gene (sak) and a second gene related to expression of fib. (C) 2002 Elsevier Science B.V. All rights reserved.