Effects of dietary copper and molybdenum on copper status, cytokine production, and humoral immune response of calves
Effects of dietary copper and molybdenum on copper status, cytokine production, and humoral immune response of calves
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DOI：
10.3168/jds.s0022-0302(98)75893-x
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发表时间：
1998-12-01
影响因子：
3.5
通讯作者：
Spears, JW
中科院分区：
文献类型：
--
作者：
Gengelbach, GP;Spears, JW
使用二十四头雄性荷斯坦犊牛来确定日粮中铜和钼对犊牛生产性能、铜状态和免疫功能的影响。给犊牛饲喂缺乏铜的代乳粉 8 周，然后在断奶后随机分配至四种处理之一：1) 对照（不补充铜或钼），2) 10 毫克铜（来自 CuSO4）/kg 干物质 (DM)（铜日粮），3) 5 毫克钼（来自 Na2MoO4）/kg DM（钼日粮），或 4) 5 毫克铜（来自 CuSO4）和 5 mg Mo（来自 Na2MoO4）/kg DM（Cu + Mo 饮食）。基础饮食是半纯化饮食，每千克干物质含有约1.1毫克铜和1.1毫克钼。在 112 天的研究中，饲喂铜和钼日粮的犊牛比饲喂对照日粮和铜+钼日粮的犊牛增重效率更高。到研究第 84 天，饲喂铜日粮的犊牛比饲喂其他三种日粮的犊牛血浆铜浓度和血浆铜蓝蛋白活性更高，并且在第 136 天肝脏铜浓度更高。饲喂对照日粮、钼日粮和铜+钼日粮的犊牛血浆和肝脏铜浓度没有差异。第 112 天时，饲喂钼日粮的犊牛的铜锌超氧化物歧化酶活性低于饲喂铜日粮的犊牛。接种后 7、14 和 21 天，饲喂钼饲料的犊牛血清抗猪红细胞总抗体（初次反应）低于饲喂铜饲料的犊牛。分离的外周血单核细胞产生的肿瘤坏死因子和白细胞介素-1 并未受到治疗的显着影响。虽然饲喂对照、钼和铜+钼日粮的犊牛血浆或肝脏铜浓度没有明显差异，但饲喂钼日粮的犊牛由于体液免疫反应和超氧化物歧化酶活性降低而出现更严重的铜缺乏症。
Twenty-four male Holstein calves were used to determine the effects of dietary Cu and Mo on performance, Cu status, and immune function of calves. Calves were fed a milk replacer that was deficient in Cu for 8 wk and then were randomly assigned after weaning to one of four treatments: 1) control (no supplemental Cu or Mo), 2) 10 mg of Cu (from CuSO4)/kg of dry matter (DM) (Cu diet), 3) 5 mg of Mo (from Na2MoO4)/kg of DM (Mo diet), or 4) 5 mg of Cu (from CuSO4) and 5 mg of Mo (from Na2MoO4)/kg of DM (Cu + Mo diet). The basal diet was a semipurified diet that contained approximately 1.1 mg of Cu and 1.1 mg of Mo/kg of DM. Calves fed the Cu and Mo diets gained weight more efficiently than those fed the control and Cu + Mo diets during the 112-d study. By d 84 of the study, calves fed the Cu diet had higher plasma Cu concentrations and plasma ceruloplasmin activities than did calves fed the other three diets and had higher Liver Cu concentrations on d 136. Plasma and liver Cu concentrations did not differ among calves fed the control, Mo, and Cu + Mo diets. At d 112, activity of Cu-Zn superoxide dismutase was lower in calves fed the Mo diet than in calves fed the Cu diet. Serum total antibodies to porcine erythrocytes (primary response) were lower in calves fed the Mo diet than in calves fed the Cu diet at 7, 14, and 21 d postinoculation. Production of tumor necrosis factor and interleukin-l by isolated peripheral blood monocytes was not significantly affected by treatment. Although no differences were apparent in plasma or Liver Cu concentrations among calves fed the control, Mo, and Cu + Mo diets, calves fed the Mo diet had a more severe Cu deficiency based on depressed humoral immune response and superoxide dismutase activity.