Cloning and characterization of T-cell lymphoma invasion and metastasis 2 (TIAM2), a novel guanine nucleotide exchange factor related to TIAM1
Cloning and characterization of T-cell lymphoma invasion and metastasis 2 (TIAM2), a novel guanine nucleotide exchange factor related to TIAM1
复制标题
DOI：
10.1006/geno.1999.5936
复制
发表时间：
1999-10-01
期刊：
影响因子：
4.4
通讯作者：
Duhl, DMJ
中科院分区：
文献类型：
--
作者：
Chiu, CY;Leng, S;Duhl, DMJ
TIAM 1是一种鸟嘌呤核苷酸交换因子，其在筛选增加T淋巴瘤细胞系侵袭性的基因中被鉴定（Habets et ab，1994，Cell 77（4）：537-549）。我们已经确定了一个基因，T细胞淋巴瘤侵袭和转移2（HGMW批准的符号TIAM 2），与TIAM 1的羧基末端区域具有显著的同一性，并将其定位于6 q25。TIAM 2在大脑中表达为类似于3.3-kb的转录物，在小脑和睾丸中表达为类似于4.4-kb的转录物。类似于4.4-kb的信息编码类似于3.3-kb的mRNA预测蛋白的较长形式，并且两者都含有与TIAM 1的Dbl同源区（70%）和Pleckstrin同源区（54%）的同源性。我们已经纯化了TIAM 2并显示其具有GDP-GTP交换活性。原位杂交显示TIAM 2在小鼠胚胎的E13.5端脑和成年小鼠大脑的大脑皮层、海马和室管膜中表达。(C)北京：科学出版社.
TIAM1 is a guanine nucleotide exchange factor that was identified in a screen for genes that increase the invasiveness of T lymphoma cell Lines (Habets et ab, 1994, Cell 77(4): 537-549). We have identified a gene, T-cell lymphoma invasion and metastasis 2 (HGMW-approved symbol TIAM2), with significant identity to the carboxyl-terminal region of the TIAM1 and mapped it to 6q25. TIAM2 is expressed as an similar to 3.3-kb transcript in cerebrum and as an similar to 4.4-kb transcript in the cerebellum and testis. The similar to 4.4-kb message encodes a longer form of the similar to 3.3-kb mRNA predicted protein, and both contain homology to the Dbl-homologous region (70%) and Pleckstrin-homologous (54%) regions of TIAM1. We have purified TIAM2 and shown it to have GDP-GTP exchange activity. In situ hybridizations demonstrate TIAM2 expression in the E13.5 telencephalon of mouse embryos and in the cerebral cortex, hippocampus, and ependyma of adult mouse brains. (C) 1999 Academic Press.