Exhaled alkanes as indices of in vivo lipid peroxidation.
Exhaled alkanes as indices of in vivo lipid peroxidation.
复制标题
呼出烷烃作为体内脂质过氧化的指标。
DOI：
10.1016/0006-2952(89)90244-x
复制
发表时间：
1989
影响因子：
5.8
通讯作者：
Ludden,TM
中科院分区：
文献类型：
--
作者：
Burk,RF;Ludden,TM
脂质过氧化被怀疑参与许多病理过程。然而，这方面的证据很难获得。一个主要问题是缺乏精确定量动物脂质过氧化速率的技术，从而准确评估实验组之间的差异。在简单的系统（例如分离的肝微粒体）中，可以通过测量底物（例如多不饱和脂肪酸）的消失或多不饱和脂肪酸分解产物（例如丙二醛）的出现来实现脂质过氧化的定量。随着实验系统变得更加复杂，这些测量结果失去了精度。细胞可以替代已过氧化的多不饱和脂肪酸，并可以代谢丙二醛。因此，这些物质的测量可能无法准确估计含有功能细胞的实验制剂中的脂质过氧化速率。当然，这包括活体动物。脂质过氧化的理想指标是用过氧化多不饱和脂肪酸以固定化学计量产生的物质，并且该物质不会代谢或从其形成和测量的隔室中损失。当 1974 年报道乙烷通过脂质过氧化反应产生时 [l]，它被认为是一个非常理想的指标。它和相关的烷烃戊烷已被广泛用于评估活体动物和更简单系统中的脂质过氧化。然而，近年来，乙烷和戊烷显然不是脂质过氧化的理想指标。本评论重点关注影响这些烷烃在脂质过氧化测量中的效用的两个过程：氧诱导的烷烃形成化学计量和代谢变化。与大多数文献一致，本评论使用戊烷特指正戊烷。
Lipid peroxidation is suspected of being involved in a number of pathological processes. Proof of this has been very difficult to obtain, however. A major problem has been the lack of a technique to quantitate precisely rates of lipid peroxidation in animals and thus to assess accurately differences between experimental groups. In simple systems such as isolated liver microsomes, quantitation of lipid peroxidation can be achieved by measuring the disappearance of a substrate, such as polyunsaturated fatty acid, or the appearance of a polyunsaturated fatty acid breakdown product, such as malondialdehyde. As experimental systems become more complex, these measurements lose their precision. Cells can replace polyunsaturated fatty acids that have been peroxidized and can metabolize malondialdehyde. Thus, measurements of these substances may not yield accurate estimates of lipid peroxidation rates in experimental preparations containing functioning cells. This includes, of course, living animals.An ideal index of lipid peroxidation would be a substance that is produced in a fixed stoichiometry with peroxidized polyunsaturated fatty acid and that is not metabolized or otherwise lost from the compartment in which it is formed and measured. When ethane was reported in 1974 to be produced by in uiuo lipid peroxidation [l], it was thought to be such an ideal index. It and the related alkane, pentane, have been used extensively to assess lipid peroxidation in living animals and in simpler systems. However, in recent years it has become apparent that ethane and pentane are not ideal indices of lipid peroxidation. This commentary focuses on two processes which affect the utility of these alkanes in the measurement of lipid peroxidation: oxygeninduced variation in their formation stoichiometry and metabolism of the alkanes. In concordance with most of the literature, this commentary uses pentane to refer specifically to n-pentane.