Tiling of the Drosophila epidermis by multidendritic sensory neurons.
Tiling of the Drosophila epidermis by multidendritic sensory neurons.
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发表时间：
2002-06
期刊：
影响因子：
4.6
通讯作者：
Wesley B. Grueber;L. Jan;Y. Jan
中科院分区：
文献类型：
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作者：
Wesley B. Grueber;L. Jan;Y. Jan
昆虫树突状树枝(Da)神经元提供了一个机会来研究不同的树突形态和树突区域是如何在发育过程中建立起来的。我们用MARCM(可抑制细胞标记镶嵌分析)系统研究了果蝇DA神经元的形态。我们发现，每个腹部半切段的15个神经元中的每一个都将树突扩散到表皮的特定区域。我们将这些神经元分为四个不同的形态类别，主要通过它们的树突分支复杂性来区分。一些课堂作业与已知的原神经基因需求相关，也与中央轴突投射相关。我们的数据表明，两个形态类别内的细胞将体壁划分为不同的、不重叠的区域，因此被组织为单独的平铺感觉系统。相比之下，不同类别细胞的树突状结构域可以广泛重叠。我们研究了星夜(Stan)(也称为火烈鸟(FMI))和红杉(SEQ)在瓷砖中的细胞自主作用。带有这些基因突变的神经元通常在侧缘和节段边缘的正常位置终止其树突场，在那里它们与相邻神经元的类似树突相遇或接近。然而，Stan突变神经元偶尔会将稀疏的分支突起发送到这些区域之外，这些突起可能会与相邻的类似树突混合。综上所述，我们的数据表明，幼虫体壁的广泛瓦片涉及不断增长的树突和尚不清楚的信号之间的相互作用，这些信号允许通过类似的树突避开。
Insect dendritic arborization (da) neurons provide an opportunity to examine how diverse dendrite morphologies and dendritic territories are established during development. We have examined the morphologies of Drosophila da neurons by using the MARCM (mosaic analysis with a repressible cell marker) system. We show that each of the 15 neurons per abdominal hemisegment spread dendrites to characteristic regions of the epidermis. We place these neurons into four distinct morphological classes distinguished primarily by their dendrite branching complexities. Some class assignments correlate with known proneural gene requirements as well as with central axonal projections. Our data indicate that cells within two morphological classes partition the body wall into distinct, non-overlapping territorial domains and thus are organized as separate tiled sensory systems. The dendritic domains of cells in different classes, by contrast, can overlap extensively. We have examined the cell-autonomous roles of starry night (stan) (also known as flamingo (fmi)) and sequoia (seq) in tiling. Neurons with these genes mutated generally terminate their dendritic fields at normal locations at the lateral margin and segment border, where they meet or approach the like dendrites of adjacent neurons. However, stan mutant neurons occasionally send sparsely branched processes beyond these territories that could potentially mix with adjacent like dendrites. Together, our data suggest that widespread tiling of the larval body wall involves interactions between growing dendritic processes and as yet unidentified signals that allow avoidance by like dendrites.