FREE D-AMINO ACIDS IN HUMAN CEREBROSPINAL-FLUID OF ALZHEIMER-DISEASE, MULTIPLE-SCLEROSIS, AND HEALTHY CONTROL SUBJECTS
FREE D-AMINO ACIDS IN HUMAN CEREBROSPINAL-FLUID OF ALZHEIMER-DISEASE, MULTIPLE-SCLEROSIS, AND HEALTHY CONTROL SUBJECTS
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DOI：
10.1007/bf02815405
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发表时间：
1994-10-01
期刊：
影响因子：
--
通讯作者：
DANIELLO, A
中科院分区：
文献类型：
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作者：
FISHER, GH;PETRUCELLI, L;DANIELLO, A
这是第一次报告的存在下，游离D-氨基酸的腰椎和脑室的人脑脊液（CSF）的个人与阿尔茨海默病（AD）与正常对照组的CSF和个人受多发性硬化症，作为一个无关的神经系统疾病。游离D-氨基酸在AD CSF中的水平显著高于正常CSF，而在受多发性硬化症影响的患者的CSF中，D-氨基酸的水平与正常对照相同。AD组脑室CSF中的总D-氨基酸含量是对照组的1.48倍（26.4 vs 17.9 nmol/ml，p = 0.025）。AD腰椎CSF中的总D-氨基酸含量是对照组的1.43倍（1.89 vs 1.32 nmol/mL，p = 0.001）。特别是D-天冬氨酸在AD脑室CSF中比正常脑室CSF高2.74倍（3.34 vs 1.22 nmol/mL，p = 0.029）。在腰椎CSF中，AD患者的D-天冬氨酸是对照组的1.5倍（0.054 vs 0.036 nmol/mL，p = 0.041）。先前我们报道了与正常脑蛋白相比，与神经元缠结相关的AD脑蛋白中D-氨基酸升高（D 'Aniello et al.，1992 c; Fisher等人，1992 a，B）。因此，CSF中存在的D-氨基酸可能来源于脑蛋白的降解。
This is the first report of the presence of free D-amino acids in lumbar and ventricular human cerebrospinal fluid (CSF) of individuals with Alzheimer disease (AD) compared with CSF of normal control subjects and with individuals affected by multiple sclerosis, as an unrelated neurologic disorder. Free D-amino acids are present at significantly higher levels in AD CSF than normal CSF, whereas in the CSF of patients affected by multiple sclerosis, D-amino acids occurs at the same level as in the normal controls. The total D-amino acid content in ventricular CSF was 1.48 times higher in the AD than controls (26.4 vs 17.9 nmol/ml, p = 0.025). The total D-amino acid content was 1.43 times higher in AD lumbar CSF than controls (1.89 vs 1.32 nmol/mL, p = 0.001). D-Aspartate in particular was 2.74 times higher in AD ventricular CSF compared to normal ventricular CSF (3.34 vs 1.22 nmol/mL, p = 0.029). In lumbar CSF, D-aspartate was 1.5 times higher in AD than controls (0.054 vs 0.036 nmol/mL, p = 0.041). Previously we reported that D-amino acids are elevated in AD brain proteins associated with neurofibrillary tangles compared to normal brain proteins (D'Aniello et al., 1992c; Fisher et al., 1992a,b). Thus, the D-amino acids present in CSF may originate from degradation of brain proteins.