Temperature-dependent vitellogenin-mRNA expression in primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes at 14 and 18 degrees C.
Temperature-dependent vitellogenin-mRNA expression in primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes at 14 and 18 degrees C.
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DOI：
10.1016/s0887-2333(00)00048-5
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发表时间：
2000-12
期刊：
影响因子：
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通讯作者：
S. Pawlowski;M. Islinger;A. Völkl;T. Braunbeck
中科院分区：
文献类型：
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作者：
S. Pawlowski;M. Islinger;A. Völkl;T. Braunbeck
为研究温度对虹鳟鱼（Oncorhynchus mykiss）原代肝细胞卵黄原蛋白基因和雌激素受体基因表达的影响，将虹鳟鱼原代肝细胞分别暴露于17β-雌二醇、双酚A和壬基酚处理48和96 h，采用非放射性斑点印迹/RNA酶保护试验和RT-PCR检测卵黄原蛋白基因mRNA表达的诱导作用。在斑点印迹/RNA酶保护试验中，双酚A和壬基酚的雌激素样作用潜力分别比17β-雌二醇低约104- 105倍和105倍。14 ° C和18°C之间的相对雌激素潜力没有显示出任何差异。相反，在18°C下，RT-PCR分析显示，与14°C相比，暴露于17β-雌二醇12和24小时后，卵黄蛋白原和雌激素受体mRNA的量增加。由于在18°C下卵黄蛋白原基因表达增加，原代肝细胞对17β-雌二醇和双酚A的敏感性可增加。
In order to study the influence of temperature on vitellogenin gene and estrogen receptor gene expression in primary hepatocytes from rainbow trout (Oncorhynchus mykiss), cells were exposed to 17β-estradiol, bisphenol-A and nonylphenol for 48 and 96 hr. Induction of vitellogenin-mRNA expression was detected in a non-radioactive dot blot/RNAse protection assay and by RT-PCR. In the dot blot/RNAse protection assay, the estrogenic potentials of bisphenol-A and nonylphenol were about 104- to 105-fold and 105-fold lower than that of 17β-estradiol, respectively. The relative estrogenic potential did not show any difference between 14 and 18°C. In contrast, at 18°C, RT-PCR analysis revealed increased amounts of vitellogenin- and estrogen receptor-mRNA after 12 and 24 hr of exposure to 17β-estradiol, if compared to 14°C. Owing to increased vitellogenin gene expression at 18°C, the sensitivity of primary hepatocytes to 17β-estradiol and bisphenol-A could be increased.