Wingless signaling directly regulates cyclin E expression in proliferating embryonic PNS precursor cells.
Wingless signaling directly regulates cyclin E expression in proliferating embryonic PNS precursor cells.
复制标题
Wingless 信号直接调节增殖胚胎 PNS 前体细胞中细胞周期蛋白 E 的表达。
DOI：
10.1016/j.mod.2008.06.006
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发表时间：
2008
影响因子：
2.6
通讯作者：
Du,Wei
中科院分区：
文献类型：
--
作者：
Deb,DilipK;Tanaka-Matakatsu,Miho;Jones,Lynn;Richardson,HelenaE;Du,Wei
细胞增殖和细胞类型规范在正常发育过程中得到协调调节。 Cyclin E 是一种关键的 G1/S 细胞周期调节因子，受多种组织特异性增强子的调节，导致果蝇发育过程中的动态表达。在这里，我们进一步表征了在发育中的周围神经系统（PNS）中调节细胞周期蛋白E表达的增强子，并表明完整的细胞周期蛋白E PNS增强子活性需要多个序列元件。我们进一步表明，Wg 信号传导通过两个保守的 TCF 结合位点对于感觉器官前体 (SOP) 细胞中细胞周期蛋白 E 的表达非常重要。阻断 Wg 信号传导并不能完全阻断 SOP 细胞形成，但可以完全阻断 SOP 细胞增殖以及随后的分化。
Cell proliferation and cell type specification are coordinately regulated during normal development. Cyclin E, a key G1/S cell cycle regulator, is regulated by multiple tissue-specific enhancers resulting in dynamic expression during Drosophila development. Here, we further characterized the enhancer that regulates cyclin E expression in the developing peripheral nervous system (PNS) and show that multiple sequence elements are required for the full cyclin E PNS enhancer activity. We further show that Wg signaling is important for the expression of cyclin E in the sensory organ precursor (SOP) cells through two conserved TCF binding sites. Blocking Wg signaling does not completely block SOP cell formation but does completely block SOP cell proliferation as well as the subsequent differentiation.