CYANINE DYE LABELING REAGENTS FOR SULFHYDRYL-GROUPS
CYANINE DYE LABELING REAGENTS FOR SULFHYDRYL-GROUPS
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DOI：
10.1002/cyto.990100103
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发表时间：
1989-01-01
期刊：
影响因子：
--
通讯作者：
WAGGONER, AS
中科院分区：
文献类型：
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作者：
ERNST, LA;GUPTA, RK;WAGGONER, AS
花青和部花青染料作为新型荧光试剂被引入，用于共价标记蛋白质和其他生物分子。这些含有碘乙酰胺官能团的染料具有高消光系数和中等量子产率。这些聚次甲基染料的一个主要优点是在合成过程中易于控制其光谱特性。含有反应性官能团的花青可以在 750 nm 范围内产生最大吸收。这一特性为涉及不同荧光探针同时多色分析的实验开辟了额外的光谱区域。花青对溶剂性质变化相对不敏感，由部花青补充，部花青是溶剂极性的敏锐指示剂。
Cyanine and merocyanine dyes are introduced as new fluorescent reagents for covalently labeling proteins and other biomolecules. These dyes, which contain iodoacetamide functional groups, have high extinction coefficients and moderate quantum yields. A major advantage of these polymethine dyes is the easy manipulation of their spectral properties during synthesis. Cyanines containing reactive functional groups can be made with absorption maxima ranging from 750 nm. This property opens additional regions of the spectrum for experiments involving the simultaneous multicolor analysis of different fluorescent probes. The cyanines, which are relatively insensitive to solvent property changes, are complemented by the merocyanines, which are keen indicators of solvent polarity.