Isolation, characterization, and molecular regulation of muscle stem cells.
Isolation, characterization, and molecular regulation of muscle stem cells.
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DOI：
10.3389/fphys.2013.00317
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发表时间：
2013
影响因子：
4
通讯作者：
Yamaguchi M
中科院分区：
文献类型：
--
作者：
Fukada S;Ma Y;Ohtani T;Watanabe Y;Murakami S;Yamaguchi M
骨骼肌具有很强的再生能力，这取决于肌肉干细胞，也称为卫星细胞。卫星细胞和/或其功能的丧失损害骨骼肌再生并导致骨骼肌动力的丧失;因此，维持卫星细胞处于静止和未分化状态的分子机制在骨骼肌生物学中具有极大的兴趣。许多研究表明卫星细胞表达的蛋白质包括Pax 7、M-cadherin、Cxcr 4、syndecan 3/4和c-met。为了进一步表征卫星细胞，我们建立了使用单克隆抗体SM/C-2.6直接分离卫星细胞的方法。使用SM/C-2.6和微阵列，我们测量了在静止卫星细胞中表达的基因，并证明Hesr 3可以补充Hesr 1产生静止卫星细胞。尽管Hesr 1或Hesr 3单基因敲除小鼠显示正常的骨骼肌表型，包括卫星细胞，但Hesr 1/Hesr 3双基因敲除小鼠显示卫星细胞数量逐渐减少，并且依赖于卫星细胞数量的再生缺陷增加。我们还观察到小鼠的遗传背景影响其骨骼肌的再生能力，并建立了一个DBA/2背景mdx小鼠系，其表型比常用的C57 BL/10-mdx小鼠严重得多。DBA/2-mdx小鼠的表型似乎也取决于卫星细胞的功能。在这篇综述中，我们根据我们的结果总结了卫星细胞的直接分离、表征和分子调控的方法。卫星细胞的再生能力和肌肉疾病的进展之间的关系也进行了总结。在最后一部分中，我们讨论了应用卫星细胞积累的科学信息治疗肌肉疾病的患者。
Skeletal muscle has great regenerative capacity which is dependent on muscle stem cells, also known as satellite cells. A loss of satellite cells and/or their function impairs skeletal muscle regeneration and leads to a loss of skeletal muscle power; therefore, the molecular mechanisms for maintaining satellite cells in a quiescent and undifferentiated state are of great interest in skeletal muscle biology. Many studies have demonstrated proteins expressed by satellite cells, including Pax7, M-cadherin, Cxcr4, syndecan3/4, and c-met. To further characterize satellite cells, we established a method to directly isolate satellite cells using a monoclonal antibody, SM/C-2.6. Using SM/C-2.6 and microarrays, we measured the genes expressed in quiescent satellite cells and demonstrated that Hesr3 may complement Hesr1 in generating quiescent satellite cells. Although Hesr1- or Hesr3-single knockout mice show a normal skeletal muscle phenotype, including satellite cells, Hesr1/Hesr3-double knockout mice show a gradual decrease in the number of satellite cells and increase in regenerative defects dependent on satellite cell numbers. We also observed that a mouse's genetic background affects the regenerative capacity of its skeletal muscle and have established a line of DBA/2-background mdx mice that has a much more severe phenotype than the frequently used C57BL/10-mdx mice. The phenotype of DBA/2-mdx mice also seems to depend on the function of satellite cells. In this review, we summarize the methodology of direct isolation, characterization, and molecular regulation of satellite cells based on our results. The relationship between the regenerative capacity of satellite cells and progression of muscular disorders is also summarized. In the last part, we discuss application of the accumulating scientific information on satellite cells to treatment of patients with muscular disorders.
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影响因子：
7.8
作者：
Asakura, Atsushi;Seale, Patrick;Girgis-Gabardo, Adele;Rudnicki, Michael A
通讯作者：
Rudnicki, Michael A
Notch 和 TGF-β 信号通路之间的串扰由 Notch 胞内结构域与 Smad3 的相互作用介导。
影响因子：
7.8
作者：
Blokzijl, A;Dahlqvist, C;Reissmann, E;Falk, A;Moliner, A;Lendahl, U;Ibáñez, CF
通讯作者：
Ibáñez, CF
影响因子：
64.5
作者：
BLAU, HM;CHIU, CP;WEBSTER, C
通讯作者：
WEBSTER, C
DOI：
10.1073/pnas.80.15.4856
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BLAU, HM;WEBSTER, C;PAVLATH, GK
通讯作者：
PAVLATH, GK
影响因子：
50.3
作者：
Acharyya, S;Butchbach, MER;Guttridge, DC
通讯作者：
Guttridge, DC