Growth factor levels in platelet-rich plasma and correlations with donor age, sex, and platelet count
Growth factor levels in platelet-rich plasma and correlations with donor age, sex, and platelet count
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DOI：
10.1054/jcms.2002.0285
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发表时间：
2002-04-01
影响因子：
3.1
通讯作者：
Hitzler, WE
中科院分区：
文献类型：
--
作者：
Weibrich, G;Kleis, WKG;Hitzler, WE
简介：富含血小板的血浆含有自体血小板生长因子，可能有希望加速牙槽骨再生。在这项研究中，分析了血小板计数和生长因子浓度。材料和方法：采用不连续细胞分离法从158名17-62岁的健康男性和55名女性中分离出富血小板血浆。分析了115份标本（按供体年龄和性别分层）的生长因子浓度和血小板计数。结果：富血小板血浆中的血小板计数（1，407，640 +/-320，100/穆尔）比供体血液中的血小板计数（266，040 +/-60，530/穆尔）高5倍。血小板衍生生长因子AB（117 +/- 63 ng/ml），转化生长因子（TGF）β-1（169 +/- 84 ng/ml）和胰岛素样生长因子（IGF）I（84 +/-23 ng/ml），而血小板衍生生长因子（PDGF）BB（10 +/-8 ng/ml）和转化生长因子β-2（0.4 +/-0.3 ng/ml）仅以少量存在。生长因子含量与全血血小板计数和富血小板血浆均无良好相关性（r（p）= 0.35）。未发现性别或年龄对血小板计数或生长因子浓度的影响（IGF-I除外）。结论：虽然富血小板血浆的生长因子含量存在很大差异，但影响因素仍值得进一步研究。此外，一种技术，其中生长因子的含量可以快速评估富含血小板的血浆可能是有益的治疗。(C)2002年欧洲颅颌面外科协会。由爱思唯尔科技有限公司出版。保留所有权利。
Introduction: Platelet-rich plasma contains autologous thrombocyte growth factors and might be promising for acceleration of dentoalveolar bone regeneration. In this study, it was analysed for platelet counts and growth factor concentrations. Material and method: Platelet-rich plasma was isolated by discontinuous cell separation from 158 healthy men and 55 women aged 17-62 years. One hundred and fifteen specimens (stratified for age and gender of the donor) were analysed for growth factor concentrations and platelet count. Results: The platelet count in platelet-rich plasma (1,407,640 +/- 320,100/mul) was 5 times higher than in donor blood (266,040 +/- 60,530/mul). Platelet-derived growth factor AB (117 +/- 63 ng/ml), transforming growth factor (TGF) beta-1 (169 +/- 84 ng/ml), and insulin-like growth factor (IGF) I (84 +/- 23ng/ml) were found in large amounts, while platelet-derived growth factor (PDGF) BB (10 +/- 8 ng/ml) and transforming growth factor beta-2 (0.4 +/- 0.3 ng/ml) were found in small amounts only. The growth factor content was not well correlated with the platelet count in whole blood nor with the platelet-rich plasma (r(p) = 0.35). No influence of gender or age on platelet count or growth factor concentrations was discovered (except IGF-I). Conclusions: While there was substantial variation in the growth factor content of platelet-rich plasma, the factors influencing this are still worthy of further investigation. Furthermore, a technique whereby the growth factor content could be rapidly assessed in platelet-rich plasma may be of therapeutic benefit. (C) 2002 European Association for Cranio-Maxillofacial Surgery. Published by Elsevier Science Ltd. All rights reserved.