Protective effects of Guizhi-Fuling-capsules on rat brain ischemia/reperfusion injury
Protective effects of Guizhi-Fuling-capsules on rat brain ischemia/reperfusion injury
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DOI：
10.1254/jphs.fp0070450
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发表时间：
2007-09-01
影响因子：
3.5
通讯作者：
Chen, Wan-Sheng
中科院分区：
文献类型：
--
作者：
Li, Tie-Jun;Qiu, Yan;Chen, Wan-Sheng
桂枝茯苓胶囊是一种由五种药用植物组成的中药制剂，包括肉桂、芍药、甘草（牡丹根）、牡丹皮（牡丹皮）、桃籽（桃核）和茯苓狼（Hoelen）对血管损伤发挥保护作用，并对谷氨酸或一硝基介导的神经元损伤具有保护作用。在本研究中，GZFLC在大鼠局灶性脑缺血和再灌注模型中的作用进行了研究。GZFLC（0.3和0.9g/kg，p.o.）局灶性脑缺血后再灌注24 h大鼠脑梗死和脑含水量明显减少，分别由模型组的31.72 ± 2.49%，84.76 ± 1.63%降至17.31 ± 3.66%，82.51 ± 1.36%和8.30 ± 3.73%，81.35 ± 1.73%。此外，对缺血脑中炎性细胞因子的分析显示，GZFLC治疗显著下调促炎细胞因子包括白细胞介素（IL）-1 β和组织坏死因子-a的表达，并显著上调抗炎细胞因子IL-10和IL-10 R在mRNA和蛋白水平上的表达。这些炎性细胞因子的血清水平也以相同的方式调节。这些结果表明，GZFLC可能是有益的治疗脑缺血再灌注损伤的部分原因是其抗炎作用。
Previous studies revealed that Guizhi-Fuling-Capsules (GZFLC), a traditional Chinese medical (Kampo) formulation composed of five kinds of medicinal plants, Cinnamomum cassia BLUME (Cinnamomi Cortex), Paeonia lactiflora PALL. (Peonies Radix), Paeonia suffruticosa ANDREWS (Moutan Cortex), Prunus persica BATSCH (Persicae Semen), and Poria cocos WOLF (Hoelen), exerts a protective effect against vascular injury and has a protective effect against glutamate- or nitro oxide-mediated neuronal damage. In the present study, the effect of GZFLC in a rat in vivo model of focal cerebral ischemia and reperfusion was investigated. Administration of GZFLC (0.3 and 0.9 g/kg, p.o.) after focal cerebral ischemia significantly decreased brain infarction and water contents in rats subjected to 2-h ischemia followed by 24-h reperfusion from 31.72 +/- 2.49%, 84.76 +/- 1.63% in the model group to 17.31 +/- 3.66%, 82.51 +/- 1.36% and 8.30 +/- 3.73%, 81.35 +/- 1.73%, respectively. Furthermore, analysis of inflammatory cytokines in ischemic brain showed that GZFLC treatment significantly down-regulated expressions of pro-inflammatory cytokines including interleukin (IL)-1 beta and tissue necrosis factor-a and markedly up-regulated expressions of anti-inflammatory cytokines IL-10 and IL-10R both in mRNA and protein levels. The serum levels of these inflammatory cytokines were also regulated the same way. These results suggested that GZFLC may be beneficial for the treatment of brain ischemia-reperfusion injury partly due to its anti-inflammatory properties.