Antibody affinity in infants after immunization with conjugated capsular polysaccharide from Haemophilus influenzae type b.
Antibody affinity in infants after immunization with conjugated capsular polysaccharide from Haemophilus influenzae type b.
复制标题
b 型流感嗜血杆菌缀合荚膜多糖免疫后婴儿的抗体亲和力。
DOI：
10.1093/infdis/162.5.1185
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Rutkowski,AF
中科院分区：
文献类型：
--
作者：
Hetherington,SV;Rutkowski,AF
用酶联免疫吸附试验（ELISA）测定了47名2 ~ 18月龄婴儿接种流感嗜血杆菌（Haemophilusinfluenzaebcapsular polysaccharide，PRP）-外膜蛋白结合物（PRP-OMP）后1个月内产生的IgG抗体的亲和力。34份血清的亲和力呈对数平均值3.2 × 105 l/mol的正态分布，但13份血清的亲和力<104 l/mol。2-6月龄和7-11月龄儿童血清的中位亲和力（1.5 × 105 l/mol）显著高于12-18月龄婴儿血清的中位亲和力（1.8 × 104 l/mol）或>18月龄婴儿血清的中位亲和力（4.2 × 104 l/mol）。来自接种与白喉类毒素结合的PRP的>18月龄儿童的血清具有6.1 × 104 l/mol的中位亲和力，相当于接种PRP-OMP的相同年龄组的亲和力。接种PRP结合疫苗的儿童可能产生亲和力极低的抗体，这一发现可能对预防侵袭性疾病具有重要意义。
The affinities of IgG antibodies toHaemophilus influenzaeb capsular polysaccharide (polyribosyl ribitol phosphate [pRP]) elicited 1 month after immunization of47 infants 2->18 months of age with a PRP-outer membrane protein conjugate (PRP-OMP) were measured by ELISA. Thirty-four sera had affinities distributed normally about a logarithmic mean of 3.2 × 105l/mol, but 13 samples had undetectable affinities <104l/mol). Median affinities of sera from children 2–6 (1.5 × l05l/mol) and 7–11 months of age (1.6 × 105l/mol) were significantly greater than the median affinities of sera from infants 12–18 (1.8 × 104l/mol) or >18 months of age (4.2 × 104l/mol). Sera from children >18 months of age vaccinated with PRP conjugated to diphtheria toxoid had a median affinity of 6.1 × 104l/mol, equivalent to that of the same age group vaccinated with PRP-OMP. Children vaccinated with PRP conjugate vaccines may produce antibodies ofvery low affinity, a finding that may have significance for protection from invasive disease.