Planococcus ruber sp nov., isolated from a polluted farmland soil sample
Planococcus ruber sp nov., isolated from a polluted farmland soil sample
复制标题
从受污染的农田土壤样品中分离出红平球菌 nov.
DOI：
10.1099/ijsem.0.001960
复制
发表时间：
2017
影响因子：
2.8
通讯作者：
Li Wen-Jun
中科院分区：
文献类型：
--
作者：
Wang Xu;Wang Zhi;Zhao Xueqiu;Huang Xing;Zhou Yu;Li Wen-Jun
从我国某污染土壤中分离到一株淡红色细菌CW 1 T，并对其进行了多相分类鉴定。CW 1 T菌株革兰氏染色阳性（或变异），球形，单极鞭毛游动，不产芽孢。在15-37 °C下观察到生长，但在10 °C或40 °C、pH 5.0-9.0和0- 5%NaCl（w/v）下未观察到生长。 系统发育分析表明菌株CW 1 T属于Planococcus属。CW 1 T与4个最接近的模式菌株Planococcus anastriticusDSM 14505 T、Planococcus halocryophilusDSM 24743 T、Planomicrobium soliXN 13 T和Planomicrobium okeanokoitesNBRC 12536 T的16 S rRNA基因序列相似性分别为97.96、97.83、97.83和97.82%。 全细胞糖包括半乳糖、核糖和葡萄糖。主要呼吸醌为MK-7，其次为MK-8，主要脂肪酸为anteiso-C15：0和anteiso-C17：0。 极性脂质由二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和磷酸糖脂组成。DNA G+C含量为48.6mol%。系统发育和化学分类学分析表明，CW 1 T菌株属于Planococcus属。然而，DNA-DNA杂交显示与动球菌属的两个最密切相关的菌株的相对较低的相关性为21.8%（DSM 14505 T）和19.6%（DSM 24743 T）。 结合基因型和表型分析，菌株CW 1 T应代表Planococcus属的一个新种，命名为Planococcus rubersp.十一是建议。模式菌株为CW 1 T（=CCTCC AB 207187 T =LMG 24442 T）。
A pale-red-pigmented bacterial strain, designated CW1T, was isolated from a polluted soil sample in China and was characterized by a polyphasic taxonomic approach. Strain CW1Twas Gram-stain-positive (or variable), coccoid, motile by a single polar flagellum and non-spore-forming. Growth was observed at 15–37 °C, but not at 10 °C or 40 °C, at pH 5.0–9.0 and with 0–5 % NaCl (w/v). Phylogenetic analysis showed that strain CW1Tbelongs to the genusPlanococcus. The 16S rRNA gene sequence similarities between CW1Tand the four most closely related type strains,Planococcus antarcticusDSM 14505T,Planococcus halocryophilusDSM 24743T,Planomicrobium soliXN13TandPlanomicrobium okeanokoitesNBRC 12536Twere 97.96, 97.83, 97.83 and 97.82 %, respectively. The whole-cell sugars contained galactose, ribose and glucose. The major respiratory quinone was MK-7 followed by MK-8, and the major fatty acids were anteiso-C15 : 0and anteiso-C17 : 0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphoglycolipid. The DNA G+C content was 48.6 mol%. The phylogenetic and chemotaxonomic analyses indicated that strain CW1Tbelongs unanimously to the genusPlanococcus. However, DNA–DNA hybridization showed relatively low relatedness of 21.8 % (DSM 14505T) and 19.6 % (DSM 24743T) with the two most closely related strains of the genusPlanococcus. Combined with the genotypic and phenotypic analysis, strain CW1Tshould represent a novel species in the genusPlanococcus, for which the namePlanococcus rubersp. nov. is proposed. The type strain is CW1T(=CCTCC AB 207187T=LMG 24442T).