Platelet-rich plasma lysate displays antibiofilm properties and restores antimicrobial activity against synovial fluid biofilms in vitro.
Platelet-rich plasma lysate displays antibiofilm properties and restores antimicrobial activity against synovial fluid biofilms in vitro.
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DOI：
10.1002/jor.24584
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发表时间：
2020-06
期刊：
影响因子：
--
通讯作者：
Schnabel LV
中科院分区：
文献类型：
--
作者：
Gilbertie JM;Schaer TP;Schubert AG;Jacob ME;Menegatti S;Ashton Lavoie R;Schnabel LV
感染性关节炎在人类和兽医临床实践中都难以治疗。最近的文献报道，金黄色葡萄球菌以及其他革兰氏阳性和革兰氏阴性分离株在人和马的滑液中形成自由漂浮的生物膜，对传统的抗菌治疗耐受。使用体外马模型，我们研究了富血小板血浆（PRP）制剂对抗滑液生物膜聚集体的能力。感染滑液，并允许在2小时内形成生物膜聚集体。收集PRP并通过血小板浓度、白细胞存在和活化或裂解加工成不同制剂。感染的滑液用不同的PRP制剂治疗，有或没有氨基糖苷类联合治疗。在处理后8小时通过连续稀释和平板计数测定细菌负荷（菌落形成单位/mL）。所有PRP制剂均显示出抗微生物特性;然而，含有较高浓度血小板且不含白细胞的制剂具有增加的抗微生物活性。与个体马相比，多匹马的PRP裂解物和PRP裂解物（PRP-L）合并进一步增加了抗微生物活性。该活性随着血浆组分的去除或血浆内蛋白水解活性的抑制而丧失。合并的PRP-L的分级鉴定出生物活性组分为阳离子和低分子量（<10 kDa）。总体而言，PRP-L与阿米卡星对氨基糖苷类耐受性生物膜聚集体表现出协同作用，对革兰氏阳性菌具有更大的活性。总之，PRP-L的使用有可能增加目前的抗菌治疗方案，这可能导致感染性关节炎相关的发病率和死亡率降低。
Infectious arthritis is difficult to treat in both human and veterinary clinical practice. Recent literature reports Staphylococcus aureus as well as other gram-positive and gram-negative isolates forming free-floating biofilms in both human and equine synovial fluid that are tolerant to traditional antimicrobial therapy. Using an in vitro equine model, we investigated the ability of platelet-rich plasma (PRP) formulations to combat synovial fluid biofilm aggregates. Synovial fluid was infected, and biofilm aggregates allowed to form over a 2-hour period. PRP was collected and processed into different formulations by platelet concentration, leukocyte presence, and activation or lysis. Infected synovial fluid was treated with different PRP formulations with or without aminoglycoside cotreatment. Bacterial load (colony-forming unit/mL) was determined by serial dilutions and plate counting at 8 hours posttreatment. All PRP formulations displayed antimicrobial properties; however, formulations containing higher concentrations of platelets without leukocytes had increased antimicrobial activity. Lysis of PRP and pooling of the PRP lysate (PRP-L) from multiple horses as compared to individual horses further increased antimicrobial activity. This activity was lost with the removal of the plasma component or inhibition of the proteolytic activity within the plasma. Fractionation of pooled PRP-L identified the bioactive components to be cationic and low-molecular weight (<10 kDa). Overall, PRP-L exhibited synergism with amikacin against aminoglycoside tolerant biofilm aggregates with greater activity against gram-positive bacteria. In conclusion, the use of PRP-L has the potential to augment current antimicrobial treatment regimens which could lead to a decrease in morbidity and mortality associated with infectious arthritis.
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影响因子：
14.2
作者：
Cobo, J.;Garcia San Miguel, L.;Ariza, J.
通讯作者：
Ariza, J.
影响因子：
4.4
作者：
Nair, Rajeshwari;Schweizer, Marin L.;Singh, Namrata
通讯作者：
Singh, Namrata
影响因子：
3.2
作者：
Gilbertie JM;Long JM;Schubert AG;Berglund AK;Schaer TP;Schnabel LV
通讯作者：
Schnabel LV
DOI：
10.1016/j.arthro.2011.10.018
发表时间：
2012-03-01
影响因子：
4.7
作者：
Boswell, Stacie G.;Cole, Brian J.;Fortier, Lisa A.
通讯作者：
Fortier, Lisa A.
影响因子：
3.7
作者：
Gilbertie, Jessica M.;Schnabel, Lauren V.;Schaer, Thomas P.
通讯作者：
Schaer, Thomas P.