Clinico-hematologic and serological comparison of Japanese and Russian strains of Theileria sergenti.
Clinico-hematologic and serological comparison of Japanese and Russian strains of Theileria sergenti.
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日本和俄罗斯塞氏泰勒菌菌株的临床血液学和血清学比较。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
T. Ishihara
中科院分区：
文献类型：
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作者：
T. Minami;T. Fujinaga;K. Furuya;T. Ishihara
本文对瑟氏泰勒虫日本株和俄罗斯株进行了临床血液学和血清学比较。通过感染感染蜱或接种感染血液，将其在4头约8月龄切除脾的小牛中传代。结果，通过蜱感染证明日本株的平均潜伏期为23天，通过接种冷冻保存的感染血液证明日本株的平均潜伏期为21天。日本株增殖期各种形态泰勒虫的检出率和平均大小分别为：逗号形和杆菌形86.8%，杆菌形2.15 × 0.73 μ m，椭圆形和球形分别为11.3%和1.97 × 1.22 μ m，球形1.46 μ m，球形1.46 μ m。四球型和无形体样型，分别为0.9%和1.0%，直径为0.54微米。日本株最高寄生率平均为11.9%，红细胞计数下降率平均为70.6%。在细胞质特别丰富的原生动物中可以看到杆状体。随着泰勒虫的繁殖，出现发热、抑郁、厌食等症状。未发生血红蛋白尿或未检测到溶血素。从俄罗斯菌株中获得的结果与从日本菌株中获得的结果基本相同。此外，日本产长角血蜱也是俄罗斯株的传播媒介。日本株与俄罗斯株的交叉补体结合试验和交叉间接荧光抗体试验结果具有良好的血清学一致性。日本株的8个株的补体结合结果无差异。
The Japanese and Russian strains of Theileria sergenti were compared clinico-hematologically and serologically. They were subjected to passage in splenectomized calves 4 approximately 8 months old by the infectation of infected ticks or the inoculation of infected blood. As a result, the average of incubation of the Japanese strain was proved to be 23 days by the tick infection and 21 days by the inoculation of infected blood stored in a frozen state. The rate of detection and the average size of various forms of Theileria in the proliferative stage of the Japanese strain were as follows: Comma-shaped and bacilli forms, 86.8%; bacilli from 2.15 x 0.73 micron; oval and spherical forms, 11.3% and 1.97 x 1.22 and 1.46 micron, respectively; tetracoccal form and anaplasma-like form, 0.9 and 1.0%, respectively, and 0.54 micron in diameter. The highest parasitic rate of the Japanese strain was 11.9% on the average, and the rate of decrease in erythrocyte count 70.6% on the average. Rods were seen in protozoa particularly rich in cytoplasm. Such symptoms as pyrexia, depression, and anorexia appeared as Theileria propagated. No hemoglobinuria occurred or no schizonts were detected. Essentially the same results were obtained from the Russian strain as from the Japanese. Besides, Haemaphysalis longicornis produced in Japan acted also as a vector for the Russian strain. There was a good serological agreement between the Japanese and the russian strain in the results of the cross complement fixation test and the cross indirect fluorescent antibody test. There was no difference in the results of the complement fixation with the eight strains of the Japanese strain.