Intracellular phosphate pools show compartmentalization of intracellular pH in turtle urinary bladder.
Intracellular phosphate pools show compartmentalization of intracellular pH in turtle urinary bladder.
复制标题
细胞内磷酸盐库显示龟膀胱中细胞内 pH 值的划分。
DOI：
10.1016/0006-291x(81)90682-3
复制
发表时间：
1981
影响因子：
3.1
通讯作者：
Burt,CT
中科院分区：
文献类型：
--
作者：
Arruda,JA;Lubansky,H;Sabatini,S;Dytko,G;Burt,CT
The urinary bladder of the fresh water turtle is capable of acidification and Na transport, in vitro, and it has been extensively used as a model of distal nephron of the kidney. In the course of measuring intracellular pH of stripped turtle bladder mucosa with phosphorus nuclear magnetic resonance, we observed the consistent presence of two inorganic phosphorus resonances under aerobic conditions, indicating the existence of a pH gradient possibly between cytosol and mitochondrion. This pH gradient was collapsed by addition of N2and could be restored by reintroduction of oxygen. These observations demonstrate the existence of a spontaneous pH gradient between cytosol and mitochondria of turtle bladder epithelial cells.
