The replicative fitness of primary human immunodeficiency virus type 1 (HIV-1) group M, HIV-1 group O, and HIV-2 isolates
The replicative fitness of primary human immunodeficiency virus type 1 (HIV-1) group M, HIV-1 group O, and HIV-2 isolates
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DOI：
10.1128/jvi.79.14.8979-8990.2005
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发表时间：
2005-07-01
影响因子：
5.4
通讯作者：
Arts, EJ
中科院分区：
文献类型：
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作者：
Ariën, KK;Abraha, A;Arts, EJ
人类免疫缺陷病毒1型（HIV-1）的主要（M）组是全球艾滋病流行的原因，而HIV-1 O组（离群值）和HIV 2型仅在西非和中非流行。HIV-2，特别是HIV-1 O组在最初的人畜共患病跳跃后未能传播，这一点还不清楚。本研究旨在检查这些人慢病毒之间的相对复制能力。用外周血单核细胞与8株HIV-2分离株、6株0组病毒和15株M组病毒（A亚型2株病毒、B亚型5株病毒、C亚型4株病毒、D亚型2株病毒和CRF01_AE亚型2株病毒）进行成对竞争实验。当在来自不同人类的外周血单核细胞中竞争时，任何亚型的HIV-1 M组分离株通常比0组或HIV-2株适合100倍。当将病毒对加入人树突状细胞中，然后与原代静止T细胞（HIV-1传播的模型）共培养时，也观察到了复制适应性的这种顺序。这些结果表明，减少复制和传播健身可能有助于低流行率和有限的地理传播的HIV-2和O组HIV-1在人群中。
The main (M) group of human immunodeficiency virus type 1 (HIV-1) is responsible for the global AIDS epidemic while HIV-1 group O (outlier) and HIV type 2 are endemic only in west and central Africa. The failure of HIV-2 and especially HIV-1 group O to spread following the initial zoonotic jumps is not well understood. This study was designed to examine the relative replicative capacities between these human lentiviruses. A pairwise competition experiment was performed with peripheral blood mononuclear cells with eight HIV-2 isolates, 6 group 0 viruses, and 15 group M viruses of subtype A (2 viruses), B (5 viruses), C (4 viruses), D (2 viruses) and CRF01_AE (2 viruses). HIV-1 group M isolates of any subtype were typically 100-fold-more fit than group 0 or HIV-2 strains when competed in peripheral blood mononuclear cells from various humans. This order in replicative fitness was also observed when virus pairs were added to human dendritic cells and then cocultured with primary, quiescent T cells, which is the model for HIV-1 transmission. These results suggest that reduced replicative and transmission fitness may be contributing to the low prevalence and limited geographical spread of HIV-2 and group O HIV-1 in the human population.