hemical Shift Images of Organelles in Leydig cells of Mice Testes
hemical Shift Images of Organelles in Leydig cells of Mice Testes
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小鼠睾丸间质细胞细胞器的化学位移图像
DOI：
10.1088/1742-6596/463/1/012047
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
and S. Tamotsu
中科院分区：
文献类型：
--
作者：
T. Ejima;Y. Neichi;M. Yanagihara;M. Kado;M. Ishino;K. Yasuda;and S. Tamotsu
用接触式显微镜观察了不同入射波长的小鼠睾丸间质细胞的软X射线透射图。在对透射图像进行归一化处理后，得到吸光度图像，并与可见的差分干涉图像进行比较。通过图像比对鉴定了一些细胞器，并从吸光度图像中获得了细胞器的吸收光谱。吸收光谱表明，根据观察到的细胞器不同，峰结构也不同。在CK吸收时，细胞器的结构和位置被清楚地识别。
Soft X-ray transmission images of Leydig cells of mice testes changing incident wavelength were observed with the use of a contact microscope. After normalization of transmission images, absorbance images were obtained and compared with a visible differential interference image. Some organelles were identified by the image comparison, and absorption spectra of the organelles were obtained from the absorbance images. The absorption spectra show that peak structures are different depending on the observed organelles. The structures and the positions of organelles were clearly identified at CK absorption.