Seewis virus: phylogeography of a shrew-borne hantavirus in Siberia, Russia.
Seewis virus: phylogeography of a shrew-borne hantavirus in Siberia, Russia.
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DOI：
10.1089/vbz.2009.0154
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发表时间：
2010-08
期刊：
影响因子：
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通讯作者：
L. N. Yashina;S. Abramov;V. Gutorov;T. Dupal;A. Krivopalov;V. Panov;G. Danchinova;V. V. Vinogradov-V.-V.-Vinograd
中科院分区：
文献类型：
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作者：
L. N. Yashina;S. Abramov;V. Gutorov;T. Dupal;A. Krivopalov;V. Panov;G. Danchinova;V. V. Vinogradov-V.-V.-Vinograd
背景汉坦病毒抗原最初是在20多年前在欧洲和俄罗斯西伯利亚捕获的欧亚鼩鼱（Sorex araneus）的组织中报道的。最近在瑞士的这种草科物种中发现了西维斯病毒（SWSV），这为研究其在俄罗斯的遗传多样性和地理分布提供了机会。方法 45 只欧亚普通鼩鼱、4 只拉克斯曼鼩鼱 (Sorex caecutiens)、3 只西伯利亚大齿鼩鼱 (Sorex daphaenodon)、9 只侏儒鼩鼱 (Sorex minutus)、28 只苔原鼩鼱 (Sorex tundrensis) 和 6 只西伯利亚鼩鼱 (Crocidura) 的肺组织西伯利亚），11年捕获 2007 年 6 月至 2008 年 9 月期间，通过逆转录聚合酶链式反应对西西伯利亚和东西伯利亚各地的汉坦病毒 RNA 进行了分析。结果在11株蜘蛛汉坦病毒、2株汉坦汉坦病毒和2株汉坦病毒中检测到的汉坦病毒L和S片段序列与SWSV密切相关，在核苷酸水平上与原型mp70株相差16.3-20.2%，在氨基酸水平上相差1.4-1.7%。核苷酸和氨基酸序列的比对和比较显示，L片段的株内差异分别为0-11.0%和0%，S片段的株内差异分别为0.2-8.5%和0%。使用邻接法、最大似然法和贝叶斯方法进行的系统发育分析显示了西伯利亚西部和东部地区 SWSV 毒株的地理特异性聚类。结论 这是西伯利亚鼩鼱传播的汉坦病毒的第一份明确报告，并证明了 SWSV 在系统发育相关的鳅属物种中的令人印象深刻的分布。 SWSV 遗传变异在 S. araneus 特定染色体种族中的共同进化和局部适应可能解释了它们的地理分布。
BACKGROUND Hantaviral antigens were originally reported more than 20 years ago in tissues of the Eurasian common shrew (Sorex araneus), captured in European and Siberian Russia. The recent discovery of Seewis virus (SWSV) in this soricid species in Switzerland provided an opportunity to investigate its genetic diversity and geographic distribution in Russia. METHODS Lung tissues from 45 Eurasian common shrews, 4 Laxmann's shrews (Sorex caecutiens), 3 Siberian large-toothed shrews (Sorex daphaenodon), 9 pygmy shrews (Sorex minutus), 28 tundra shrews (Sorex tundrensis), and 6 Siberian shrews (Crocidura sibirica), captured in 11 localities in Western and Eastern Siberia during June 2007 to September 2008, were analyzed for hantavirus RNA by reverse transcription-polymerase chain reaction. RESULTS Hantavirus L and S segment sequences, detected in 11 S. araneus, 2 S. tundrensis, and 2 S. daphaenodon, were closely related to SWSV, differing from the prototype mp70 strain by 16.3-20.2% at the nucleotide level and 1.4-1.7% at the amino acid level. Alignment and comparison of nucleotide and amino acid sequences showed an intrastrain difference of 0-11.0% and 0% for the L segment and 0.2-8.5% and 0% for the S segment, respectively. Phylogenetic analysis, using neighbor-joining, maximum-likelihood, and Bayesian methods, showed geographic-specific clustering of SWSV strains in Western and Eastern Siberia. CONCLUSIONS This is the first definitive report of shrew-borne hantaviruses in Siberia, and demonstrates the impressive distribution of SWSV among phylogenetically related Sorex species. Coevolution and local adaptation of SWSV genetic variants in specific chromosomal races of S. araneus may account for their geographic distribution.