Adjuvant Pluronic F68 Is Compatible with a Plant Root-Colonizing Probiotic, Pseudomonas chlororaphis O6
Adjuvant Pluronic F68 Is Compatible with a Plant Root-Colonizing Probiotic, Pseudomonas chlororaphis O6
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DOI：
10.3390/agrochemicals3010001
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发表时间：
2023-12
期刊：
影响因子：
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通讯作者：
Amanda R. Streeter;Anthony Cartwright;Mohammad Zargaran;Anagha Wankhade;Anne J. Anderson;D. Britt
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文献类型：
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作者：
Amanda R. Streeter;Anthony Cartwright;Mohammad Zargaran;Anagha Wankhade;Anne J. Anderson;D. Britt
植物益生菌正被越来越多地用于最大限度地提高大田作物的产量和质量。绿假单胞菌O6(PcO6)是一种具有益生菌活性的植物根部定殖菌。这种细菌产生一系列代谢物，包括一组在植物保护中起作用的吩嗪类化合物。本文报道了PcO_6对非离子三嵌段共聚物表面活性剂Pluronic F68的响应。这种Pluronic在真核细胞中显示出膜的“愈合”活性，并提高了冷冻保存的恢复率。该产品获得FDA批准，并作为佐剂应用于农业、医药和生物技术的配方中。在25℃下，0.1和1.0g/L F68不抑制PcO6在溶源发酵液上的生长，10g/L使PcO6生长减少，100g/L F68对PcO6生长有显著抑制作用，较高剂量时胶束形成可能是抑制生长的原因。F68对吩嗪的产量没有影响，但F68的表面活性导致细菌在0.5%琼脂上定植。将细胞暴露在荧光素标记的F68中，产生强烈的荧光，对洗涤稳定，表明Pluronic与细菌直接结合。然而，悬浮在0.1%或1%F68中的PcO6细胞在3次冷冻(−)20°C/解冻循环后，既没有保护作用，也没有发现损害。这些发现表明，F68可以兼容用于农业配方，而对PcO6等益生菌几乎没有影响。
Plant probiotic bacteria are being increasingly used to maximize both the productivity and quality of field crops. Pseudomonas chlororaphis O6 (PcO6) is a plant root colonizer with probiotic activities. This bacterium produces an array of metabolites, including a group of phenazines that are functional in plant protection. The paper reports responses of PcO6 to a nonionic triblock copolymer surfactant, Pluronic F68. This Pluronic exhibits membrane “healing” activity and improves cryopreservation recovery in eukaryotic cells. The product is FDA-approved and is applied as an adjuvant in formulations used in agriculture, medicine, and biotechnology. Growth of PcO6 on lysogeny broth at 25 °C was unhindered by 0.1 and 1.0 g/L F68, reduced at 10 g/L, and with significant inhibition at 100 g/L F68; micelle formation could account for inhibited growth at higher doses. Phenazine production was not changed by F68, whereas the surfactant activity of F68 induced the spread of bacterial colonization on 0.5% agar. Exposure of cells to fluorescein-labeled F68 resulted in intense fluorescence, stable to washing, showing a direct association of the Pluronic with the bacterium. However, neither protection nor harm was found for PcO6 cells suspended in either 0.1% or 1% F68 after three freeze (−20 °C)/thaw cycles. These findings suggest that F68 could be compatible for use in agricultural formulations with little effect on probiotics such as PcO6.