GENETICALLY STRUCTURED MODELS FOR IAC PROMOTER-OPERATOR FUNCTION IN THE ESCHERICHIA-COLI CHROMOSOME AND IN MULTICOPY PLASMIDS - IAC OPERATOR FUNCTION
GENETICALLY STRUCTURED MODELS FOR IAC PROMOTER-OPERATOR FUNCTION IN THE ESCHERICHIA-COLI CHROMOSOME AND IN MULTICOPY PLASMIDS - IAC OPERATOR FUNCTION
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DOI：
10.1002/bit.260261115
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发表时间：
1984-01-01
影响因子：
3.8
通讯作者：
BAILEY, JE
中科院分区：
文献类型：
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作者：
LEE, SB;BAILEY, JE
基于已知的分子相互作用，建立了一个数学模型来定量描述乳糖操纵子在大肠杆菌中的表达调控。coli染色体和多拷贝质粒中。该模型的遗传结构使得影响lac启动子-操纵子处转录起始的核苷酸序列变化影响1个或非常少的直接对应的模型参数。该模型模拟了染色体乳糖操纵子的功能，与以前的实验测量许多lacI和lacO突变体系统，以及二倍体细胞携带F“乳糖附加体。模拟结果清楚地表明，随着质粒拷贝数的增加，克隆的乳糖操纵基因调控的损失，与实验趋势一致。这类模型在设计DNA，生物体和反应器的克隆基因表达的精确调控的重要性进行了讨论。
A mathematical model based on known molecular interactions was formulated to describe quantitatively regulation of expression of the lactose (lac) operon in the E. coli chromosome and in multicopy plasmids. This model was genetically structured such that a nucleotide sequence change affecting transcription initiation at the lac promoter-operator influences 1 or very few directly corresponding model parameters. The model simulates chromosomal lac operon function in good agreement with previous experimental measurements for many lacI and lacO mutant systems as well as for diploid cells which carry F''lac episomes. Simulation results clearly show the loss of cloned lac operator regulation as the plasmid copy number increases, in agreement with experimental trends. The importance of this class of models in designing DNA, organisms and reactors for precise regulation of cloned gene expression is discussed.