INDUCTION OF NITROTYROSINE-LIKE IMMUNOREACTIVITY IN THE LOWER MOTOR-NEURON OF AMYOTROPHIC-LATERAL-SCLEROSIS
INDUCTION OF NITROTYROSINE-LIKE IMMUNOREACTIVITY IN THE LOWER MOTOR-NEURON OF AMYOTROPHIC-LATERAL-SCLEROSIS
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DOI：
10.1016/0304-3940(95)12039-7
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发表时间：
1995-10-20
影响因子：
2.5
通讯作者：
ITOYAMA, Y
中科院分区：
文献类型：
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作者：
ABE, K;PAN, LH;ITOYAMA, Y
用免疫组织化学方法对肌萎缩侧索硬化症(ALS)散发性患者和正常对照组脊髓进行了铜/锌超氧化物歧化酶(SOD)和硝基酪氨酸(NT)免疫组织化学染色。肌萎缩侧索硬化症患者运动神经元的铜/锌超氧化物歧化酶免疫反应阳性率与对照组无明显差异，而神经降压素在肌萎缩侧索硬化症患者运动神经元中的免疫反应强度较高，而对照组运动神经元的铜锌超氧化物歧化酶免疫反应强度较低。肌萎缩侧索硬化症患者运动神经元的轴突也有这种染色，而对照组则未见。这些结果提示ALS脊髓运动神经元中蛋白质酪氨酸残基的硝化作用上调。
Spinal cords of sporadic cases with amyotrophic lateral sclerosis (ALS) and normal controls were immunohistochemically examined using antibodies for Cu/Zn superoxide dismutase (SOD) and nitrotyrosine (NT). Immunoreactivity for Cu/Zn SOD of the motor neurons was not different between the ALS and controls, In contrast, immunoreactivity for NT was densely detected in motor neurons of ALS but was not or was only minimally detected in those of controls. The staining was also found in the axons of motor neurons of ALS, but was not found in the controls. These results suggest that nitration of protein-tyrosine residue is upregulated in motor neurons of the spinal cord of ALS.