Human growth hormone: A multigene family
Human growth hormone: A multigene family
复制标题
人类生长激素：多基因家族
DOI：
10.1016/0092-8674(82)90144-1
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发表时间：
1982
期刊：
影响因子：
64.5
通讯作者：
H. Goodman
中科院分区：
文献类型：
--
作者：
D. Moore;M. A. Conkling;H. Goodman
利用克隆的着丝粒功能DNA（CEN 3和CEN 11）作为标记探针，研究了酵母染色体III和XI着丝粒区域的染色质结构。当用微球菌核酸酶消化来自分离的细胞核的染色质并将所得DNA片段电泳分离并印迹到硝酸纤维素滤膜上时，着丝粒核小体亚单位被分解成比来自大部分染色质的那些明显更明显的梯状结构。通过绘制着丝粒染色质内的精确核酸酶切割位点，揭示了CEN序列的220-250 bp的离散保护区，其两侧是高度核酸酶敏感的位点。
We have examined the chromatin structure of the centromere regions of chromosomes III and XI in yeast by using cloned functional centromere DNAs (CEN3 and CEN11) as labeled probes. When chromatin from isolated nuclei is digested with micrococcal nuclease and the resulting DNA fragments separated electrophoretically and blotted to nitrocellulose filters, the centromeric nucleosomal sub-units are resolved into significantly more distinct ladders than are those from the bulk of the chromatin. A discrete protected region of 220–250 bp of CEN sequence flanked by highly nuclease-sensitive sites was revealed by mapping the exact nuclease cleavage sites within the centromeric chromatin.