Mitochondrial membrane studies using impedance spectroscopy with parallel pH monitoring.
Mitochondrial membrane studies using impedance spectroscopy with parallel pH monitoring.
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DOI：
10.1371/journal.pone.0101793
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zagozdzon-Wosik W
中科院分区：
文献类型：
--
作者：
Padmaraj D;Pande R;Miller JH Jr;Wosik J;Zagozdzon-Wosik W
设计了一种生物微机电系统（BioMEMS）装置，用于研究线粒体功能障碍研究中重要的互补线粒体参数。线粒体功能障碍与许多疾病有关，包括糖尿病、肥胖、心力衰竭和衰老，因为这些细胞器在能量产生、细胞信号传导和细胞凋亡中起着关键作用。ATP的合成是由穿过线粒体内膜的电势和由穿过线粒体膜的质子通量引起的pH值差异驱动的。我们开发了一种工具来研究线粒体的离子活性与电介质测量（阻抗谱）并行，以更好地了解线粒体膜的特性。该BioMEMS芯片包括：1)用于线粒体阻抗研究的电极，设计为双探针和四探针结构，以优化在宽频率范围内的操作；2)用于质子研究的离子敏感场效应晶体管电子传递链，并可能监测其他离子，如钠，钾和钙。我们使用解偶联剂使线粒体膜去极化并破坏离子平衡。电介质谱反应阻抗值相应增加，指向线粒体膜电位的变化。电学模型用于描述线粒体样品的复杂阻抗频率依赖性和膜对整体阻抗变化的贡献。结果表明，电介质光谱可以作为研究膜电位的一种工具。由此可以得出结论，研究与线粒体生物能量学相关的电化学参数可以提供归因于这些细胞器的各种异常的重要信息。
A biological microelectromechanical system (BioMEMS) device was designed to study complementary mitochondrial parameters important in mitochondrial dysfunction studies. Mitochondrial dysfunction has been linked to many diseases, including diabetes, obesity, heart failure and aging, as these organelles play a critical role in energy generation, cell signaling and apoptosis. The synthesis of ATP is driven by the electrical potential across the inner mitochondrial membrane and by the pH difference due to proton flux across it. We have developed a tool to study the ionic activity of the mitochondria in parallel with dielectric measurements (impedance spectroscopy) to gain a better understanding of the properties of the mitochondrial membrane. This BioMEMS chip includes: 1) electrodes for impedance studies of mitochondria designed as two- and four-probe structures for optimized operation over a wide frequency range and 2) ion-sensitive field effect transistors for proton studies of the electron transport chain and for possible monitoring other ions such as sodium, potassium and calcium. We have used uncouplers to depolarize the mitochondrial membrane and disrupt the ionic balance. Dielectric spectroscopy responded with a corresponding increase in impedance values pointing at changes in mitochondrial membrane potential. An electrical model was used to describe mitochondrial sample’s complex impedance frequency dependencies and the contribution of the membrane to overall impedance changes. The results prove that dielectric spectroscopy can be used as a tool for membrane potential studies. It can be concluded that studies of the electrochemical parameters associated with mitochondrial bioenergetics may render significant information on various abnormalities attributable to these organelles.
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影响因子：
5.5
作者：
Buckler, KJ;Vaughan-Jones, RD
通讯作者：
Vaughan-Jones, RD
影响因子：
10.6
作者：
Duchen, Michael R.
通讯作者：
Duchen, Michael R.
影响因子：
4.8
作者：
Dzbek, Jaroslaw;Korzeniewski, Bernard
通讯作者：
Korzeniewski, Bernard
DOI：
10.1016/0006-291x(62)90189-4
发表时间：
1962-01-01
影响因子：
3.1
作者：
HEYTLER, PG;PRICHARD, WW
通讯作者：
PRICHARD, WW
影响因子：
10.8
作者：
Brennan, Jonathan P.;Southworth, Richard;Shattock, Michael J.
通讯作者：
Shattock, Michael J.