Honey bee males and queens use glandular secretions to enhance sperm viability before and after storage
Honey bee males and queens use glandular secretions to enhance sperm viability before and after storage
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DOI：
10.1016/j.jinsphys.2009.01.012
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发表时间：
2009-06-01
影响因子：
2.2
通讯作者：
Baer, Boris
中科院分区：
文献类型：
--
作者：
den Boer, Susanne P. A.;Boomsma, Jacobus J.;Baer, Boris
体内受精需要在卵子受精之前将活精子从雄性转移到雌性。雄性和雌性都通过向精子提供腺体分泌物来协助授精过程，据推测腺体分泌物含有维持精子活力的蛋白质子集。在这里，我们发现，蜜蜂（Apis mellifera）的雄性副腺分泌物是精液的主要贡献者，可以提高精子的存活率。我们进一步证明，男性副腺分泌的蛋白质部分对于实现精子存活的最大影响确实很重要。精子储存后，蜂后还会向精子提供受精囊腺的分泌物，我们发现这些分泌物对精子的活力具有相当的积极影响。 SDS 凝胶显示，副腺分泌和精囊液分泌的蛋白质组谱几乎不重叠，这表明男性和女性分别在射精和最终精子储存期间使用不同的蛋白质来增强精子活力。 (C) 2009 Elsevier Ltd. 保留所有权利。
Internal fertilization requires live sperm to be transferred from male to female before egg fertilization. Both males and females assist the insemination process by providing sperm with glandular secretions, which have been inferred to contain subsets of proteins that maintain sperm viability. Here we show that in the honeybee (Apis mellifera) secretions of the male accessory glands, the major contributors towards seminal fluid, enhance sperm survival. We further demonstrate that the protein fraction of the male accessory gland secretion is indeed important for achieving the maximal effect on sperm survival. After sperm storage, the queens also provide sperm with secretions from spermathecal glands and we show that these secretions have a comparable positive effect on sperm viability. SDS gels show that the proteomic profiles of accessory gland secretion and spermathecal fluid secretion hardly overlap, which suggests that males and females use different proteins to enhance sperm viability during, respectively, ejaculation and final sperm storage. (C) 2009 Elsevier Ltd. All rights reserved.