Expression of aquaporin 3 (AQP3) in normal and neoplastic lung tissues
Expression of aquaporin 3 (AQP3) in normal and neoplastic lung tissues
复制标题
DOI：
10.1016/j.humpath.2006.07.015
复制
发表时间：
2007-01-01
期刊：
影响因子：
3.3
通讯作者：
Katoh, Ryohei
中科院分区：
文献类型：
--
作者：
Liu, Ya Lan;Matsuzaki, Toshiyuki;Katoh, Ryohei
水通道蛋白3 （AQP3）是水和其他小溶质的膜通道，在流体稳态中起重要作用。为了研究AQP3在正常肺组织和肿瘤肺组织中的表达，我们采用免疫组化、Western blotting和逆转录聚合链反应的方法对149例肺癌组织和2种细胞系进行了研究。在正常肺组织中，AQP3在支气管基底细胞、肺泡11型细胞、细支气管上皮细胞和粘膜下腺分泌细胞中均有免疫组化表达。84例肺腺癌中有59例（70.2%）表达AQP3。鳞状细胞癌和大细胞癌的阳性率较低（分别为35.8%和13.4%）。AQP3在小细胞癌、多形性癌、转移性结肠腺癌中未见表达。在腺癌中，所有细支气管肺泡亚型肿瘤均检测到AQP3。乳头状亚型AQP3的阳性比例也高于腺泡型和固体粘蛋白亚型。此外，AQP3的表达与腺癌的肿瘤分化及临床分期有关。Western blotting和逆转录聚合链反应分析证实了AQP3蛋白和信使RNA在肺腺癌细胞系和组织中的表达。我们得出结论，AQP3在正常呼吸道中广泛表达，并在维持水分稳态中发挥重要作用。此外，肺癌尤其是腺癌也能产生AQP3，这可能与其功能和/或生物学性质有关，但AQP3在肺癌中表达的具体机制尚不清楚。(c) 2007年Elsevier Inc.出版
Aquaporin 3 (AQP3) acts as the membrane channel of water and other small solutes and plays a major role in fluid homeostasis. To investigate the expression of AQP3 in normal and neoplastic lung tissues, we studied a series of 149 lung carcinoma tissues and 2 cell lines by immunohistochemistry, Western blotting, and reverse transcriptase-polymerise chain reaction. In normal lung tissues, immunohistochemical expression of AQP3 was demonstrated in bronchial basal cells, alveolar type 11 cells, bronchiolar epithelial cells, and secretory cells of submucosal glands. In lung carcinomas, AQP3 expression was observed in 59 (70.2%) of 84 adenocarcinomas. Squamous cell carcinoma and large cell carcinoma had rather low positive ratios (35.8% and 13.4%, respectively). No AQP3 expression was demonstrated in small cell carcinoma, pleomorphic carcinoma, or metastatic colon adenocarcinoma. In adenocarcinomas, AQP3 was detected in all tumors of bronchioloalveolar subtype. Papillary subtype also showed a higher positive ratio of AQP3 compared with that in acinar and solid with mucin subtypes. In addition, AQP3 expression was related to tumor differentiation and clinical stage in adenocarcinomas. Western blotting and reverse transcriptase-polymerise chain reaction analyses confirmed the expression of AQP3 protein and messenger RNA in cell lines and tissues of lung adenocarcinoma. We conclude that AQP3 is widely expressed in the normal respiratory tract and can play an important role in the maintenance of water homeostasis. In addition, lung carcinomas, especially adenocarcinomas, can produce AQP3, possibly in connection with their functional and/or biological nature, although the detailed mechanism of AQP3 expression in lung carcinomas remains to be clarified. (c) 2007 Published by Elsevier Inc.