Screening of genes associated with dedifferentiation and effect of LBD29 on pericycle cells in Arabidopsis thaliana

Screening of genes associated with dedifferentiation and effect of LBD29 on pericycle cells in Arabidopsis thaliana
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拟南芥去分化相关基因的筛选及LBD29对中柱鞘细胞的影响

DOI:
10.1007/s10725-010-9495-4
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发表时间:
2010-07
期刊:
Plant Growth Regulation: An International Journal On Plant Growth and Development
影响因子:
--
通讯作者:
Zhu, Jian
Zhu, Jian
中科院分区:
其他
文献类型:
--
作者:
Liu, Hai-liang;Wang, Guang-Chao;Feng, Zhenhua;Zhu, Jian

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脱分化是植物发育过程中的一个重要细胞过程。植物组织离体培养的成功与否取决于外植体的脱分化和再分化。然而,去分化的分子基础还不清楚。为了阐明脱分化的机制,利用基因芯片技术研究了拟南芥叶柄脱分化过程中转录组的变化。全球基因表达谱的分析,使我们能够确定关键的发育基因,在去分化过程中表现出差异表达。LBD 29被筛选为调控拟南芥侧根发生的候选基因之一。讨论了LBD 29/ASL 16基因在影响周鞘细胞脱分化和侧根形成中的作用。
Dedifferentiation is an important cellular process in plant development. Successful in vitro culture of plant tissues for micropropagation and transgenic plant development depend on dedifferentiation and redifferentiation of explants. However, the molecular basis of dedifferentiation is not well understood. To elucidate the dedifferentiation mechanism, microarray analysis was carried out to study the transcriptome changes associated with dedifferentiation in the petiole of Arabidopsis thaliana. Analysis of global gene expression profiles allowed us to identify key developmental genes that showed differential expression during the dedifferentiation process. LBD29 was selected as one of the candidate genes that regulate the process of lateral root initiation in Arabidopsisthaliana. The role of the LBD29/ASL16 gene in effecting dedifferentiation of pericycle cells and lateral root formation is discussed.
DOI: 10.1016/0047-7206(77)90003-6
发表时间: 1977
期刊: Micron
影响因子: 2.4
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