Class II restriction in mice to the malaria candidate vaccine ring infected erythrocyte surface antigen (RESA) as synthetic peptides or as expressed in recombinant vaccinia.
Class II restriction in mice to the malaria candidate vaccine ring infected erythrocyte surface antigen (RESA) as synthetic peptides or as expressed in recombinant vaccinia.
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小鼠对疟疾候选疫苗环感染红细胞表面抗原（RESA）作为合成肽或在重组痘苗中表达的II类限制。
DOI：
10.4049/jimmunol.142.11.4012
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发表时间：
1989
影响因子：
4.4
通讯作者：
R. Anders
中科院分区：
文献类型：
--
作者：
A. Lew;C. Langford;D. Pye;S. Edwards;L. Corcoran;R. Anders
研究了疟疾候选疫苗环感染的E表面抗原（RESA）重复区对应的三种肽的免疫应答。在小鼠注射这些肽没有载体的抗体反应和淋巴细胞刺激被发现限于某些MHC II类单倍型。携带IAk的小鼠是对所有三种肽的强应答者。携带IAd的小鼠仅对3'重复肽、八聚体和四聚体有强应答。携带Is或Iq的小鼠对任何重复肽均无反应。值得注意的是，对牛痘中表达的整个RESA的抗体应答的遗传限制模式表明，除了三个重复序列外，没有其他表位。因为只有一种II类单倍型（即，k）五个测试者中有两个对该肽有强烈反应，k和d）响应于八聚体或四聚体，可能难以在大多数或所有人中实现针对RESA的良好免疫应答。
The immune response to three peptides corresponding to the repeat regions of the malaria candidate vaccine ring infected E surface Ag (RESA) were studied. Both antibody responses and lymphocyte stimulation in mice injected with these peptides without carrier were found to be restricted to certain MHC class II haplotypes. Mice bearing IAk were strong responders to all three peptides. Mice bearing IAd were strong responders only to the 3' repeat peptides, the octamer and tetramer. Mice bearing Is or Iq did not respond to any repeat peptides. Remarkably, the pattern of genetic restriction of the antibody response to the entire RESA as expressed in vaccinia indicated that there were no other epitopes besides the three repeats. Because only one class II haplotype (i.e., k) out of five tested responded strongly to this peptide and only two out of five (i.e., k and d) responded to the octamer or tetramer, it may be difficult to achieve a good immune response against RESA in most or all humans.