HPLC method validated for the simultaneous analysis of cichoric acid and alkamides in Echinacea purpurea plants and products
HPLC method validated for the simultaneous analysis of cichoric acid and alkamides in Echinacea purpurea plants and products
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DOI：
10.1021/jf026158f
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发表时间：
2003-11-19
影响因子：
6.1
通讯作者：
Cornett, C
中科院分区：
文献类型：
--
作者：
Molgaard, P;Johnsen, S;Cornett, C
建立了一种反相高效液相色谱法测定紫锥菊中咖啡酸衍生物菊苣酸和醇酰胺类化合物的方法。该方法包括一个提取程序，其中亲水性酚类和亲脂性烷基酰胺从样品中释放，然后通过分析HPLC程序定量测定这些化合物。该方法是第一个验证用于在同一程序中测定这两组化合物的方法。柚皮素已被用作内标，因为提取物中不存在其他黄烷酮，并且它不会干扰任何正在研究的化合物。对丹麦种植的植物材料的分析表明，在丹麦种植化学质量非常高的植物是可能的。丹麦市场上的一系列国际草药产品显示出令人惊讶的质量差异，不一定反映标签上的产品信息。
A reversed-phase high-performance liquid chromatography (HPLC) method has been developed to determine caffeic acid derivatives, for example, cichoric acid, and alkamides in plant parts and herbal products of Echinacea purpurea. The method consists of an extraction procedure whereby the hydrophilic phenolics as well as the lipophilic alkamides are released from the samples, followed by the analytical HPLC procedure for quantitative determination of these compounds. The method is the first one validated for the determination of these two groups of compounds in the same procedure. Naringenin has been used as an internal standard, as no other flavanones are present in the extract and it does not interfere with any of the compounds under investigation. Analysis of Danish-grown plant material shows that it is possible to raise plants of a very high chemical quality in Denmark. A selection of international herbal products available on the Danish market show surprisingly variable quality, not necessarily reflecting the product information given on the labels.