SIMPLE AND SENSITIVE COLORIMETRIC METHOD FOR THE DETERMINATION OF LONG-CHAIN FREE FATTY-ACIDS IN SUBCELLULAR ORGANELLES
SIMPLE AND SENSITIVE COLORIMETRIC METHOD FOR THE DETERMINATION OF LONG-CHAIN FREE FATTY-ACIDS IN SUBCELLULAR ORGANELLES
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DOI：
10.1016/0003-2697(79)90093-9
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发表时间：
1979-01-01
影响因子：
2.9
通讯作者：
CHAN, SHP
中科院分区：
文献类型：
--
作者：
NIXON, M;CHAN, SHP
本文介绍了一种测定长链游离脂肪酸（FFA）的比色法。用氯仿：庚烷：甲醇从亚细胞器中提取游离脂肪酸。用二苯碳酰二肼比色法测定游离脂肪酸铜皂。采用本改进的方法有3个优点：灵敏度提高了约10%。2-比Falholt等先前的方法高出一倍;与目前可用的繁琐方法相比，完成测定花费的时间更少;并且牛血清白蛋白（一种已知的FAA结合蛋白）的存在不会干扰测定方法。该测定显示在10-130 nmol FFA范围内呈线性响应。[大鼠肝脏]线粒体中游离脂肪酸的回收率为99%。
A colorimetric assay for the determination of long-chain free fatty acids (FFA) is described. The FFA were extracted from subcellular organelles with chloroform:heptane:methonal. The Cu soaps of FFA were colorimetrically determined with diphenylcarbazide. There are 3 advantages to employing the present modified procedure: the sensitivity is increased .apprx. 2-fold over the previous procedure of Falholt et al; it takes less time to complete the assay compared to the tedious procedures currently available; and the presence of bovine serum albumin, a known FAA-binding protein, does not interfere with the assay procedure. The assay shows a linear response over the range of 10-130 nmol of FFA. The recovery of free fatty acids from [rat liver] mitochondria is 99%.