A new densovirus isolated from the mosquito Culex pipiens (Diptera: Culicidae)
A new densovirus isolated from the mosquito Culex pipiens (Diptera: Culicidae)
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DOI：
10.1016/s0168-1702(00)00128-3
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发表时间：
2000-03-01
期刊：
影响因子：
5
通讯作者：
Bergoin, M
中科院分区：
文献类型：
--
作者：
Jousset, FX;Baquerizo, E;Bergoin, M
我们已经从实验室品系的尖音库蚊，其中异常的幼虫死亡率发生了一个小的二十面体无包膜DNA病毒共享浓核病毒（DNVs）的主要生物学和生物物理特性。与先前从伊蚊物种分离的DNV，即AneDNV和AalDNV不同（Afanasiev，B.N.，Galyov，E.E.，Buchatsky，L.P.，Kozlov，Y.V.，1991.伊蚊浓核病毒的核苷酸序列和基因组结构。Virology 185，323-336; Boublik，Y.，Jousset，F. X.，Bergoin，M.，1994.对埃及伊蚊幼虫致病的白纹伊蚊细小病毒（AaPV）的完整核苷酸序列和基因组结构。Virology 200，752-763），这种名为CpDNV的蚊子DNV具有6 kb的基因组，分别以化学计量比作为“正”和“负”链进行双链化。CpDNV和AalDNV基因组之间缺乏序列同源性，并且它们的衣壳多肽之间缺乏抗原交叉反应性，这表明这两种蚊子DNV在遗传学上是遥远的。相反，CpDNV似乎与Junonia coenia浓核病毒（JcDNV）在血清学和基因组水平上都相关。(C)2000 Elsevier Science B. V.保留所有权利。
We have isolated from a laboratory strain of Culex pipiens in which abnormal larval mortality occurred a small icosahedral nonenveloped DNA virus sharing the main biological and biophysical properties of densoviruses (DNVs). Unlike DNVs isolated previously from Aedes species, i.e. the AneDNV and the AalDNV (Afanasiev, B.N., Galyov, E.E., Buchatsky, L.P., Kozlov, Y.V., 1991. Nucleotide sequence and genomic organization of Aedes densonucleosis virus. Virology 185, 323-336; Boublik, Y., Jousset, F.-X., Bergoin, M., 1994. Complete nucleotide sequence and genomic organization of the Aedes albopictus Parvovirus (AaPV) pathogenic for Aedes aegypti larvae. Virology 200, 752-763), this mosquito DNV named CpDNV possesses a genome of 6 kb separately encapsidated in stoichiometric proportion as 'plus' and 'minus' strands. The lack of sequence homology between the CpDNV and the AalDNV genome and of antigenic cross-reactivity between their capsid polypeptides indicates that these two mosquito DNVs are phylogenetically distant. In contrast, the CpDNV appears to be related to the Junonia coenia densovirus (JcDNV) at both serological and genomic levels. (C) 2000 Elsevier Science B.V. All rights reserved.