Effects of Astragalus polysaccharide on the erythroid lineage and microarray analysis in K562 cells
Effects of Astragalus polysaccharide on the erythroid lineage and microarray analysis in K562 cells
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DOI：
10.1016/j.jep.2009.11.013
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发表时间：
2010-02-03
影响因子：
5.4
通讯作者：
Fu, Su-Zhen
中科院分区：
文献类型：
--
作者：
Yang, Min;Qian, Xin-Hua;Fu, Su-Zhen
民族药理学相关性：黄芪多糖（Astragalus polysaccharide，APS）是从黄芪（Astragalus membranaceus）中提取的一种多糖，具有免疫调节、抗炎、血管保护等多种生物活性。本研究的目的：利用基因芯片技术研究黄芪多糖对K562细胞向红系分化的影响及其作用机制。材料与方法：联苯胺染色，半定量RT-PCR，采用Western blot和基因芯片技术检测黄芪多糖诱导红系细胞分化的作用及基因表达的变化结果：联苯胺染色法检测到13.2%的K562阳性细胞，200 μ g/ml APS在72 h时诱导率最高。G γ-mRNA表达和胎儿血红蛋白合成显著上调。基因芯片分析显示31个基因表达上调，108个基因表达下调。这些差异表达基因通常调节蛋白质结合、细胞代谢过程、细胞增殖和转录激活因子活性。γ-珠蛋白基因表达上调，与红系分化相关的基因LMO 2、Runx 1、GTF 2 I表达上调，Bklf、Eklf、EPHB 4、Sp1表达下调。我们的研究表明，APS通过调节LMO 2、Klf 1、Klf 3、Runx 1、EphB 4和Sp1等基因，对红系分化显示出有效的活性，增加K562细胞中的γ-珠蛋白mRNA表达和胎儿血红蛋白合成。皇冠版权所有（C）2009由爱思唯尔爱尔兰有限公司出版。保留所有权利。
Ethnopharmacological relevance: Astragalus polysaccharide (APS), obtained from Astragalus membranaceus, displays a range of activities in many systems, including the promotion of immune responses, anti-inflammation, and the protection of vessels. It possesses potent pharmacological activity on differentiation to the erythroid lineage.Aim of the study: To investigate the effects of APS on the erythroid differentiation and the mechanism of action by microarray analysis in K562 cells.Materials and methods: Benzidine staining, semi-quantitative RT-PCR, Western blot and microarray methods were used to survey the effects of APS on inducing erythroid differentiation and the changes of gene expression profile in K562 cells.Results: Of the 13.2% positive cells detected by benzidine staining, the induction was the highest with 200 mu g/ml APS on 72 h. G gamma-mRNA expression and fetal hemoglobin synthesis were significantly up-regulated. Microarray analysis showed that 31 genes were up-regulated and 108 genes were down-regulated. These differential expression genes generally regulate protein binding, cellular metabolic process, the cell proliferation, and transcriptional activator activity. The gamma-globin gene was up-regulated, the genes related with erythroid differentiation such as LMO2, Runx1 and GTF2I were up-regulated, while Bklf, Eklf, EPHB4 and Sp1 were down-regulated.Conclusions: Our studies indicate that APS indicate potent activities on the erythroid differentiation by modulating genes of LMO2, Klf1, Klf3, Runx1, EphB4 and Sp1, increasing gamma-globin mRNA expression and fetal hemoglobin synthesis in K562 cells. Crown Copyright (C) 2009 Published by Elsevier Ireland Ltd. All rights reserved.