Direct: Attachment of Oligonucleotides to Quantum Dot Interfaces
Direct: Attachment of Oligonucleotides to Quantum Dot Interfaces
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DOI：
10.1021/cm2021593
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发表时间：
2011-11-22
影响因子：
8.6
通讯作者：
Maye, Mathew M.
中科院分区：
文献类型：
--
作者：
Han, Hyunjoo;Zylstra, Joshua;Maye, Mathew M.
描述了一种用于将单链寡核苷酸（ssDNA）直接连接到量子点（qdot）界面的简单官能化策略。该方法利用组氨酸介导的相转移协议，导致量子点在水性缓冲液中具有高胶体稳定性。弱结合的组氨酸包封促进与硫醇化ssDNA和多组氨酸标记的蛋白质的单层交换。通过FRET分析探测在qdot界面处的成功生物修饰。测量的适度FRET效率表明DNA处于延伸构象，这是直接附着提供的高表面覆盖的结果。
A straightforward functionalization strategy for the direct attachment of single-stranded oligonucleotides (ssDNA) to quantum dot (qdot) interfaces is described. The approach takes advantage of a histidine-mediated phase transfer protocol that results in qdots with high colloidal stability in aqueous buffers. The weakly bound histidine encapsulation facilitates monolayer exchanged with both thiolated ssDNA and polyhistidine-tagged proteins. The successful biomodification at the qdot interface was probed by FRET analysis. The modest FRET efficiencies measured suggest the DNA to be in an extended conformation that is the result of high surface coverage that the direct attachment provides.