Indole-porphyrin hybrids produced by metagenomics
Indole-porphyrin hybrids produced by metagenomics
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通过宏基因组学产生吲哚-卟啉杂种
DOI：
10.1016/j.bmcl.2013.04.076
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发表时间：
2013-07-01
影响因子：
2.7
通讯作者：
Abe, Ikuro
中科院分区：
文献类型：
--
作者：
Yang, Xiao-Long;Wakimoto, Toshiyuki;Abe, Ikuro
新的吲哚-卟啉杂合分子是从大肠杆菌中分离的，该大肠杆菌携带来自日本海绵盘皮海绵的宏基因组DNA。吲哚被附加到由插入DNA编码的硬卟啉发色团的反应性乙烯基取代基上。因此，异源表达的卟啉和内源吲哚之间的嵌合途径在大肠杆菌中产生了新的吲哚共轭手性卟啉。杆菌(c)2013爱思唯尔有限公司保留所有权利。
New indole-porphyrin hybrid molecules were isolated from Escherichia coli harboring metagenomic DNA from the Japanese marine sponge Discodermia calyx. The indole was appended to the reactive vinyl substituent of the harderoporphyrin chromophore, encoded by the insert DNA. Thus, the chimeric pathway between the heterologously expressed porphyrins and the endogenous indole generated new indole-conjugated chiral porphyrins in E. coli. (c) 2013 Elsevier Ltd. All rights reserved.