Compost-bulking agents reduce the reservoir of antibiotics and antibiotic resistance genes in manures by modifying bacterial microbiota.
Compost-bulking agents reduce the reservoir of antibiotics and antibiotic resistance genes in manures by modifying bacterial microbiota.
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DOI：
10.1016/j.scitotenv.2018.08.212
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发表时间：
2019-02
期刊：
影响因子：
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通讯作者：
Jin Zhang;Hui Lin;Junwei Ma;Wanchun Sun;Yuyi Yang;Xin Zhang
中科院分区：
文献类型：
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作者：
Jin Zhang;Hui Lin;Junwei Ma;Wanchun Sun;Yuyi Yang;Xin Zhang
木屑，稻壳，蘑菇渣通常被用作堆肥过程中的膨胀剂，这项工作评估了它们的潜力，提高去除抗生素和抗生素耐药基因（ARGs）的温度一致的条件下。结果表明，所有堆肥填充剂的添加都提高了猪粪中抗生素的去除率，提高率为14.9%-33.4%;但它们对减少鸡粪中抗生素残留的作用较小，其中氟喹诺酮类抗生素是主要抗生素，部分原因是填充剂对氟喹诺酮类抗生素降解的促进作用较弱。添加填充剂在某种程度上阻碍了猪粪便中ARGs的减少，而添加填充剂的鸡粪中ARGs的总相对丰度明显降低。在三种填充剂中，木屑是最有效的增强剂，以去除抗生素在这两个粪肥，和稻壳表现出最好的性能，减少ARGs在鸡粪。抗生素，ARGs和细菌群落之间的关系随后被描绘。变形菌在添加填充剂对猪粪中抗生素和ARGs的影响中起着关键作用。特别是，增加黄单胞菌科贡献了很大的促进抗生素的降解，以及高水平的ARGs在猪粪与锯末添加。相比之下，在鸡粪中添加填充剂的优势细菌家族的变化并不足以有效地提高抗生素的去除，但在很大程度上影响了ARGs的丰度。添加稻壳对类芽孢杆菌和葡萄球菌的大量减少与添加稻壳的鸡粪中ARGs的减少密切相关。
Sawdust, rice husk, and mushroom residues are commonly used as bulking agents during manure composting; this work evaluated their potentiality for enhancing the removal of antibiotics and antibiotic resistance genes (ARGs) under a temperature-consistent condition. Results indicated that the addition of all the compost-bulking agents increased antibiotic removal in swine manure with increasing rates of 14.9%–33.4%; however they showed less effect on the reduction of residual antibiotics in chicken manure where fluoroquinolone (FQs) antibiotics are the dominant antibiotics, partly owing to the weak promoting effects of bulking agents on FQs degradation. The addition of bulking agents somehow hindered the reduction of ARGs within swine manure, whereas there were obvious reductions in the total relative abundance of ARGs in chicken manure with bulking agents added. Among the three bulking agents, sawdust was the most efficient enhancer for antibiotic removal in both manures, and rice husk exhibited the best performance on ARGs reduction in chicken manure. The relationship between antibiotics, ARGs and bacteria communities was subsequently delineated. Proteobacteria was proposed to play key roles on the effect of bulking agent addition on antibiotics and ARGs in swine manure. Particularly, the increased Xanthomonadaceae contributed much to the promoted antibiotic degradation as well as the high level of ARGs in swine manure with sawdust added. By contrast, the changes in dominant bacterial families by the addition of bulking agents into chicken manure were not strong enough to effectively enhance antibiotic removal, but largely influenced the ARGs abundance. The large reductions of Paenibacillaceae and Staphylococcaceae by rice husk addition were closely related to the reduced ARGs in chicken manure with rice husk added.