Profound mycobiome differences between segregated mouse colonies do not influence Th17 responses to a newly introduced gut fungal commensal
Profound mycobiome differences between segregated mouse colonies do not influence Th17 responses to a newly introduced gut fungal commensal
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DOI：
10.1016/j.fgb.2019.03.001
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发表时间：
2019-06-01
影响因子：
3
通讯作者：
Iliev, Iliyan D.
中科院分区：
文献类型：
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作者：
Doron, Itai;Leonardi, Irina;Iliev, Iliyan D.
肠道菌群失调可对几种炎症性疾病的结果产生负面影响，表明菌群在影响宿主免疫力方面的作用。然而，尚不清楚肠道真菌生物群组合物是否可以产生影响对新引入的肠道真菌的免疫应答的免疫环境。使用ITS 1深度测序，我们评估了从杰克逊（JAX）获得的C57 BL/6 J小鼠的真菌生物群结构，或在我们自己的小鼠设施（WCM-CE）的专用房间的受控环境中饲养了几代的C57 BL/6 J小鼠。我们发现来自这些隔离小鼠群体的C57 BL/6 J小鼠具有显著不同的真菌生物群。为了评估真菌生物群组成是否可以影响对鼠胃肠道外来真菌定殖的免疫应答，我们用人大肠杆菌定殖JAX和WCM-CE小鼠。白色念珠菌，并测量肠道中的Th 17应答。我们发现，独立于真菌组成，小鼠产生强烈的Th 17反应，胃肠道C。白色念珠菌定植。我们的数据表明，不同的小鼠菌落可以携带显着不同的真菌。然而，在该实验环境中，对新引入的机会性真菌的强Th 17应答被有效地诱导，与真菌生物群背景无关。
Gut mycobiota dysbiosis can negatively impact the outcome of several diseases of inflammatory origin, suggesting a role of the mycobiota in influencing the host immunity. However, it is unknown whether the gut mycobiota composition can create an immune environment that would influence the immune response to a newly introduced intestinal fungus. Using ITS1 deep sequencing, we evaluated the mycobiome structure of C57BL/6J mice acquired from Jackson (JAX) or bred in a controlled environment at a dedicated room in our own mouse facility (WCM-CE) for several generations. We found that C57BL/6J mice from these segregated mouse colonies harbor dramatically different mycobiota. To assess whether the mycobiota make up can influence immune responses to colonization with a fungus foreign to the murine GI tract, we colonized JAX and WCM-CE mice with the human commensal C. albicans and measured Th17 responses in the gut. We found that independent of mycobiota composition, mice produced strong Th17 responses to gastrointestinal C. albicans colonization. Our data suggest that different mouse colonies can carry dramatically different mycobiota. Nevertheless, strong Th17 responses to a newly introduced opportunistic commensal fungus are potently induced independent of the mycobiota background in this experimental setting.