Effect of type-2 astrocytes on the viability of dorsal root ganglion neurons and length of neuronal processes.
Effect of type-2 astrocytes on the viability of dorsal root ganglion neurons and length of neuronal processes.
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DOI：
10.4103/1673-5374.125339
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发表时间：
2014-01-15
影响因子：
6.1
通讯作者：
Cao Q
中科院分区：
文献类型：
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作者：
Fan C;Wang H;Chen D;Cheng X;Xiong K;Luo X;Cao Q
2型星形胶质细胞在中枢神经系统损伤修复中的作用仍知之甚少。在这项研究中，使用一个相对简单的培养条件，在体外，2型星形胶质细胞，分化的少突胶质细胞前体细胞与骨形态发生蛋白-4，共培养背根神经节神经元。我们研究了从少突胶质细胞前体细胞分化的2型星形胶质细胞对背根神经节神经元存活和生长的影响。结果表明，少突胶质前体细胞和2型星形胶质细胞共培养后，背根神经节神经元的数量高于单独培养时，但低于神经元与1型星形胶质细胞共培养。单个神经元的最长突起和所有突起的长度在单独培养的神经元中最短，其次是与2型星形胶质细胞共培养的神经元，然后是与少突胶质前体细胞共培养的神经元，最长的是与1型星形胶质细胞共培养的神经元。这些结果表明，与2型星形胶质细胞共培养可以增加神经元存活率和突起长度。但与1型星形胶质细胞和少突胶质前体细胞相比，2型星形胶质细胞对背根节神经元生长的促进作用较弱。
The role of type-2 astrocytes in the repair of central nervous system injury remains poorly understood. In this study, using a relatively simple culture condition in vitro, type-2 astrocytes, differentiated from oligodendrocyte precursor cells by induction with bone morphogenetic protein-4, were co-cultured with dorsal root ganglion neurons. We examined the effects of type-2 astrocytes differentiated from oligodendrocyte precursor cells on the survival and growth of dorsal root ganglion neurons. Results demonstrated that the number of dorsal root ganglion neurons was higher following co-culture of oligodendrocyte precursor cells and type-2 astrocytes than when cultured alone, but lower than that of neurons co-cultured with type-1 astrocytes. The length of the longest process and the length of all processes of a single neuron were shortest in neurons cultured alone, followed by neurons co-cultured with type-2 astrocytes, then neurons co-cultured with oligodendrocyte precursor cells, and longest in neurons co-cultured with type-1 astrocytes. These results indicate that co-culture with type-2 astrocytes can increase neuronal survival rate and process length. However, compared with type-1 astrocytes and oligodendrocyte precursor cells, the promotion effects of type-2 astrocytes on the growth of dorsal root ganglion neurons were weaker.
影响因子：
--
作者：
Miller RH
通讯作者：
Miller RH