Preparation of Spherical Nucleic Acid Nanoparticles Containing a Self-immolative Poly(carbamate) Core
Preparation of Spherical Nucleic Acid Nanoparticles Containing a Self-immolative Poly(carbamate) Core
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DOI：
10.2116/analsci.20scn06
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发表时间：
2021-05-01
影响因子：
1.6
通讯作者：
Kikuchi, Akihiko
中科院分区：
文献类型：
--
作者：
Fukumoto, Shione;Kawade, Mami;Kikuchi, Akihiko
我们以氨基苯乙醇为重复单元，将末端活化的聚氨基甲酸酯(PC)衍生物与端胺基寡聚DNA偶联到PC-寡聚DNA载体上，制备了一种新型的球形核酸，其核心结构为自焚的聚氨基甲酸酯(PC)。动态光散射测量表明，其流体力学直径为107 nm，粒径分布较窄。一种含有氨基苯甲醇的荧光单体可用于PC-寡DNA的合成，以增强PC在各种外界刺激下像多米诺骨牌一样分解的荧光发射。
We prepared a novel spherical nucleic acid, containing a core structure of self-immolative poly(carbamate) (PC), with aminobenzyl alcohol as a repeating unit, by conjugating an end-activated PC derivative with an amine-terminated oligoDNA on a solid support for PC-oligoDNA. Dynamic light-scattering measurements revealed a hydrodynamic diameter of 107 nm with a narrow size distribution. A fluorescent monomer with aminobenzyl alcohol is available for PC-oligoDNA synthesis to enhance the fluorescence emission by a domino-like disassembly of PC in response to various external stimuli.