Kinesin molecular motor Eg5 functions during polypeptide synthesis.
Kinesin molecular motor Eg5 functions during polypeptide synthesis.
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DOI：
10.1091/mbc.e11-03-0211
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发表时间：
2011-09
影响因子：
3.3
通讯作者：
Saunders WS
中科院分区：
文献类型：
--
作者：
Bartoli KM;Jakovljevic J;Woolford JL Jr;Saunders WS
微管马达Eg5因其在有丝分裂过程中的功能而闻名。研究表明，在间期，Eg5与核糖体结合，是有效合成蛋白质所必需的。与激酶相关的分子马达Eg5在细胞分裂中起作用，促进纺锤体组装。我们发现，在间期期间，Eg5与核糖体相关，是最佳新生多肽合成所必需的。当Eg5被抑制时，核糖体在体外不再与微管结合，核糖体转运速度减慢，并且在完整细胞中积累，表明多肽合成过程中存在延伸或终止缺陷。这些结果表明，分子马达Eg5与核糖体结合，提高了翻译效率。
The microtubule motor Eg5 is well known for its functions during mitosis. It is shown that during interphase, Eg5 associates with ribosomes and is required for efficient protein synthesis. The kinesin-related molecular motor Eg5 plays roles in cell division, promoting spindle assembly. We show that during interphase Eg5 is associated with ribosomes and is required for optimal nascent polypeptide synthesis. When Eg5 was inhibited, ribosomes no longer bound to microtubules in vitro, ribosome transit rates slowed, and polysomes accumulated in intact cells, suggesting defects in elongation or termination during polypeptide synthesis. These results demonstrate that the molecular motor Eg5 associates with ribosomes and enhances the efficiency of translation.