Persistence and infectivity of Zika virus in semen after returning from endemic areas: Report of 5 cases
Persistence and infectivity of Zika virus in semen after returning from endemic areas: Report of 5 cases
复制标题
DOI：
10.1016/j.jcv.2017.10.006
复制
发表时间：
2017-11-01
影响因子：
8.8
通讯作者：
Arsuaga, M.
中科院分区：
文献类型：
--
作者：
Garcia-Bujalance, S.;Gutierrez-Arroyo, A.;Arsuaga, M.
背景：关于从疫区回国的有症状旅行者精液中寨卡病毒的持久性和传染性的数据有限，在无症状病例中的数据更是少之又少。目的：我们调查了5例寨卡病毒感染从流行区返回西班牙的患者精液中寨卡病毒的持久性和传染性。精液中通过聚合酶链式反应、部分测序和细胞培养检测到寨卡病毒。结果：在发病第14天至第96天的精液标本中，用聚合酶链式反应检测到寨卡病毒RNA(亚洲系)。两名患者的精液病毒培养在病程第30天和69天通过病毒传播呈寨卡病毒阳性。系统发育分析强烈提示一对从马尔代夫回来的夫妇存在男性向女性的性传播。结论：这一系列病例证实，在感染寨卡病毒后三个月的精液中可以检测到寨卡病毒RNA。精液样本的病毒培养显示，长时间的传染性可能导致寨卡病毒的性传播。
Background: There are limited data about the persistence and infectivity of Zika virus in semen of symptomatic travelers returning from endemic areas and even less data in asymptomatic cases.Objective: We investigated the persistence and infectivity of ZIKA virus in semen in five patients with Zika virus infection returning to Spain from endemic areas.Study design: We evaluated the epidemiological, clinical and virological characteristic of the five patients. In semen we detected ZIKA virus by PCR, partial sequencing and cell culture. We also performed phylogenetic analysis.Results: We detected Zika virus RNA (Asian lineage) by PCR in semen samples from day 14th to day 96th since the day of illness onset. Semen viral culture was positive for Zika virus in two patients at days of illness 30 and 69 by virus propagation. Phylogenetic analysis strongly suggested male to female sexual transmission in a couple returning from Maldives.Conclusion: This case series confirms that Zika virus RNA can be detected in semen up to three months after infection. Viral culture of semen samples shows prolonged infectivity that can lead to sexual transmission of Zika virus.