Microtiter plate bioassay to monitor the interference of antibiotics with the lipid II cycle essential for peptidoglycan biosynthesis
Microtiter plate bioassay to monitor the interference of antibiotics with the lipid II cycle essential for peptidoglycan biosynthesis
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DOI：
10.1016/j.mimet.2008.05.002
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发表时间：
2008-09-01
影响因子：
2.2
通讯作者：
Stein, Torsten
中科院分区：
文献类型：
--
作者：
Burkard, Michael;Stein, Torsten
协调适当遗传反应的特定药物传感系统确保微生物在抗生素存在下的存活。我们报告的开发和应用的微量滴定板为基础的生物测定的抗生素干扰肽聚糖生物合成所必需的脂质II周期的鉴定。一种枯草芽孢杆菌报告菌株，其特异性地感测脂质II -干扰细胞壁生物合成胁迫（T. Mascher，S.L Zimmer，T. A. Smith和J. Helmann，由枯草芽孢杆菌的细胞包膜应力感应双组分系统LiaRS调节的抗生素诱导型启动子; Antimicrob.2005，第102页。探员Chemother第48卷（2004年），pp. 2888-2896）在不同硫醚抗生素存在下进行分析。我们可以显示出报告细胞对B产生的羊毛硫抗生素枯草杆菌素的作用的剂量依赖性细胞壁生物合成应激。枯草杆菌、表皮葡萄球菌或S.在琼脂平板和基于液体培养的测定中，令人惊讶的是，先前已知特异性结合生物膜的磷脂酰乙醇胺的肉桂霉素（Streptomyces cinnamoneus cinnamoneus）也引起强烈的细胞壁生物合成应激。我们的研究结果表明，我们的系统可用于筛选目的，例如发现新的细胞壁生物合成抑制剂。(C)2008年由Elsevier B. V.出版。
Specific drug-sensing systems that coordinate appropriate genetic responses assure the survival of Microorganisms in the presence of antibiotics. We report on the development and application of a microtiter plate-based bioassay for the identification of antibiotics interfering with the lipid II cycle essential for peptidoglycan biosynthesis. A Bacillus subtilis reporter strain sensing specifically lipid II - interfering cell wall biosynthesis stress (T. Mascher, S.L Zimmer, T.-A. Smith and J. Helmann, Antibiotic-inducible promoter regulated by the cell envelope stress-sensing two-component system LiaRS of Bacillus subtilis; Antimicrob. Agents Chemother., Vol 48 (2004) pp. 2888-2896) was analyzed in the presence of different lantibiotics. We could show dose-dependent cell wall biosynthesis stress of reporter cells in response to the action of the lantibiotics subtilin produced by B. subtilis, epidermin and gallidermin of Staphylococcus epidermidis or S. gallinarum, respectively, in both, agar-plate and liquid culture-based assays. Surprisingly, also cinnamycin of Streptomyces cinnamoneus cinnamoneus), previously known to bind specifically to phosphatidylethanolamin of biological membranes, provoked strong cell wall biosynthetic stress. Our results show that our system can be used for screening purposes, for example to discover novel inhibitors of cell wall biosynthesis. (C) 2008 Published by Elsevier B.V.