Abscisic acid employs NRP-dependent PIN2 vacuolar degradation to suppress auxin-mediated primary root elongation in Arabidopsis
Abscisic acid employs NRP-dependent PIN2 vacuolar degradation to suppress auxin-mediated primary root elongation in Arabidopsis
复制标题
脱落酸利用 NRP 依赖性 PIN2 液泡降解来抑制拟南芥中生长素介导的初生根伸长
DOI：
10.1111/nph.17783
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发表时间：
2021
期刊：
影响因子：
9.4
通讯作者：
Liu Xinqi
中科院分区：
文献类型：
--
作者：
Wu Yanying;Chang Yue;Luo Liming;Tian Wenqi;Gong Qingqiu;Liu Xinqi
How plants balance growth and stress adaptation is a long‐standing topic in plant biology. Abscisic acid (ABA) induces the expression of the stress‐responsive Asparagine Rich Protein (NRP), which promotes the vacuolar degradation of PP6 phosphatase FyPP3, releasing ABI5 transcription factor to initiate transcription. Whether NRP is required for growth remains unknown.We generated annrp1 nrp2double mutant, which had a dwarf phenotype that can be rescued by inhibiting auxin transport. Insufficient auxin in the transition zone and over‐accumulation of auxin at the root tip was responsible for the short elongation zone and short‐root phenotype ofnrp1 nrp2.The auxin efflux carrier PIN2 over‐accumulated innrp1 nrp2and became de‐polarized at the plasma membrane, leading to slower root basipetal auxin transport. Knock‐out ofPIN2suppressed the dwarf phenotype ofnrp1 nrp2. Furthermore, ABA can induce NRP‐dependent vacuolar degradation of PIN2 to inhibit primary root elongation. FyPP3 also is required for NRP‐mediated PIN2 turnover.In summary, in growth condition, NRP promotes PIN2 vacuolar degradation to help maintain PIN2 protein concentration and polarity, facilitating the establishment of the elongation zone and primary root elongation. When stressed, ABA employs this pathway to inhibit root elongation for stress adaptation.
