The rck/p54 candidate proto-oncogene product is a 54-kilodalton D-E-A-D box protein differentially expressed in human and mouse tissues.
The rck/p54 candidate proto-oncogene product is a 54-kilodalton D-E-A-D box protein differentially expressed in human and mouse tissues.
复制标题
DOI：
--
复制
发表时间：
1995-08
期刊：
影响因子：
11.2
通讯作者：
Yukihiro Akao;Osamu Marukawa;Hiroshi Morikawa;Keiichi Nakao;Mikako Kamei;Takahisa Hachiya;Yoshihide Tsujimoto
中科院分区：
文献类型：
--
作者：
Yukihiro Akao;Osamu Marukawa;Hiroshi Morikawa;Keiichi Nakao;Mikako Kamei;Takahisa Hachiya;Yoshihide Tsujimoto
RCK 基因是 B 细胞淋巴瘤细胞系 RC-K8 中 t(11;14) (q23;q32) 易位 11q23 上的靶基因，通过 Northern 和 Western 印迹分析研究了 RCK 基因的表达。 RCK 基因产物是 D-E-A-D 盒蛋白/RNA 解旋酶家族的成员。通过使用 Northern 印迹分析，RCK 基因的 7.5 kb 转录物显示在人和小鼠组织中普遍表达。产生了针对 RCK 基因产物的多克隆抗体，并在人和小鼠组织中检查了 RCK 基因表达模式。通过蛋白质印迹分析，两种不同的多克隆抗 rck 抗体在大多数人类和小鼠组织中检测到了一种名为 rck/p54 的特定 54 千道尔顿产物。然而，rck/p54 在人脑中的含量非常低，并且在腰肌和肺组织中检测不到，尽管 RCK mRNA 大量存在于这些组织中。有趣的是，来自 rck/p54 低表达或无表达的组织（例如神经母细胞瘤、胶质母细胞瘤、横纹肌肉瘤和肺癌细胞系）的恶性转化人类细胞产生适量的 rck/p54 蛋白，表明 rck/p54 在肿瘤发生中发挥作用。此外，通过使用激光显微镜进行免疫染色和亚细胞分级，将 rck/p54 蛋白定位于细胞质。
Expression of the RCK gene, which is a target gene on 11q23 of the t(11;14) (q23;q32) translocation in the B-cell lymphoma cell line RC-K8, was studied by Northern and Western blot analyses. The RCK gene product is a member of the D-E-A-D box protein/RNA helicase family. With the use of Northern blot analysis, a 7.5-kb transcript of the RCK gene was shown to be expressed ubiquitously in human and mouse tissues. Polyclonal antibodies against the RCK gene product were raised, and the RCK gene expression pattern was examined in human and mouse tissues. Two different polyclonal anti-rck antibodies detected a specific 54-kilodalton product named rck/p54 in the majority of human and mouse tissues tested by Western blot analysis. However, rck/p54 was shown to be very low in the human brain and was not detectable in lumbar muscle and lung tissues, although RCK mRNA is abundantly present in these tissues. It is of interest that malignant transformed human cells arising from tissues with low or no expression of rck/p54, such as neuroblastoma, glioblastoma, rhabdomyosarcoma, and lung cancer cell lines, produced a moderate amount of rck/p54 protein, suggesting that rck/p54 plays a role in tumorigenesis. In addition, the rck/p54 protein was localized to cytoplasm by immunostaining with the use of laser microscopy and by subcellular fractionation.